Prevalence of biofilm formation in clinical isolates of Candida species causing bloodstream infection
Prevalence of biofilm formation in clinical isolates of Candida species causing bloodstream infection
复制标题
DOI：
10.1111/myc.12014
复制
发表时间：
2013-05-01
期刊：
影响因子：
4.9
通讯作者：
Romling, Ute
中科院分区：
文献类型：
--
作者：
Pannanusorn, Srisuda;Fernandez, Victor;Romling, Ute
念珠菌属是医院内侵袭性感染的第四大常见原因。生物被膜的形成被认为是念珠菌的一个毒力因子。共检出白色念珠菌243株，念珠菌81株。glabrata、33 C. parapsilosis、14 C. dubliniensis、8 C. tropicalis，8 C. lusitaniae、5 C. krusei和1 C.评价引起血流感染的薄壳菌分离物的生物膜形成。通过XTT试验估计代谢活性并通过光学和扫描电子显微镜观察，对与人血清预孵育的硅橡胶上形成的生物膜进行定量。40%的C.白色念珠菌形成生物被膜率为88.7%，非白色念珠菌形成生物被膜率为88.7%（P
Candida species are the fourth most common cause of nosocomial invasive infections. Biofilm formation is recognised as one virulence factor of Candida species. A total of 243 Candida albicans, 81 C. glabrata, 33 C. parapsilosis, 14 C. dubliniensis, 8 C. tropicalis, 8 C. lusitaniae, 5 C. krusei and 1 C. pelliculosa isolates causing bloodstream infections were evaluated for biofilm formation. The biofilm formed on silicone elastomer preincubated with human serum was quantified by estimation of the metabolic activity through XTT assay and visualised by light and scanning electron microscopy. Forty per cent of the C. albicans isolates formed biofilm compared to 88.7% of the non-albicans Candida isolates (P