Temporal changes in NCAM immunoreactivity during taste cell differentiation and cell lineage relationships in taste buds
Temporal changes in NCAM immunoreactivity during taste cell differentiation and cell lineage relationships in taste buds
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DOI：
10.1093/chemse/bji031
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发表时间：
2005-05-01
期刊：
影响因子：
3.5
通讯作者：
Hino, A
中科院分区：
文献类型：
--
作者：
Miura, H;Kato, H;Hino, A
神经细胞粘附分子（NCAM）是味蕾中的III型细胞标志物。为了阐明味蕾中Mash 1表达细胞的细胞类型，使用NCAM免疫组织化学和原位杂交的组合，在成年小鼠的Mash 1表达味觉细胞中与味觉调节蛋白和T1 r3表达细胞进行比较，检查NCAM的表达。大约98%的Mash 1表达细胞是NCAM免疫阳性（IP），这表明Mash 1表达细胞应被归类为III型细胞。出乎意料的是，也发现了小亚群的gustducin和T1 r3表达细胞是NCAM-IP，与以前在大鼠中的免疫组织化学研究相矛盾，其中gustducin-IP细胞在II型细胞中被观察到，而II型细胞不具有NCAM免疫反应性。对发育中味蕾的检查显示，NCAM-IP细胞在表达味觉调节蛋白和T1 r3的细胞中的比例随时间发生变化;在出生后0.5天，NCAM-IP细胞在这些基因表达细胞中的比例接近90%，在发育过程中显着下降。相反，大多数Mash 1表达细胞在整个发育过程中表现出恒定的NCAM免疫反应性。此外，BrdU标记实验表明，Mash 1表达细胞的分化先于成年小鼠味蕾中的gustducin和T1 r3表达细胞的分化。这些结果表明，T1 r3和gustducin表达细胞NCAM-IP在细胞分化的开始，和NCAM免疫反应性gustducin和T1 r3表达细胞可能仍然从以前的发展阶段表达Mash 1。
Neural cell adhesion molecule (NCAM) is a type III cell marker in the taste buds. In order to clarify the cell type of Mash1-expressing cells in taste buds, expression of NCAM was examined in Mash1-expressing taste cells of adult mice in comparison with gustducin- and T1r3-expressing cells, using a combination of NCAM immunohistochemistry and in situ hybridization. About 98% of Mash1-expressing cells were NCAM immunopositive (IP), suggesting that Mash1-expressing cells should be categorized as type III cells. Unexpectedly, small subsets of gustducin- and T1r3-expressing cells were also found to be NCAM-IP, contradicting previous immunohistochemical studies in rats, in which gustducin-IP cells were observed specifically in type II cells, which do not have NCAM immunoreactivity. Examinations of developing taste buds showed temporal changes in the ratio of NCAM-IP cells in gustducin- and T1r3-expressing cells; the ratio of NCAM-IP cells in these gene-expressing cells were similar to 90% at 0.5 days after birth and decreased markedly during development. In contrast, the majority of Mash1-expressing cells showed constant NCAM immunoreactivity throughout development. In addition, BrdU-labeling experiments showed that the differentiation of Mash1-expressing cells precedes those of gustducin- and T1r3-expressing cells in taste buds of adult mice. These results suggest that T1r3- and gustducin-expressing cells are NCAM-IP at the beginning of cell differentiation, and that NCAM immunoreactivity in gustducin- and T1r3-expressing cells might remain from the previous developmental stage expressing Mash1.