Morphometry of human leukocytes.
Morphometry of human leukocytes.
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DOI：
10.1182/blood.v56.5.866.bloodjournal565866
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发表时间：
1980-11
期刊：
影响因子：
20.3
通讯作者：
G. Schmid-Schönbein;Y. Y. Shih-Y.;S. Chien
中科院分区：
文献类型：
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作者：
G. Schmid-Schönbein;Y. Y. Shih-Y.;S. Chien
为了建立白细胞功能的定量模型，需要单个白色细胞的几个形态计量学参数。这些参数包括未变形状态下细胞及其细胞核的直径、体积和膜面积。使用体视学方法从人白色血细胞（中性粒细胞、淋巴细胞、单核细胞和嗜酸性粒细胞）随机切片的透射电子显微镜获得这些数量。为了估计由于制备透射电子显微镜细胞而可能出现的伪影，与光学显微镜进行了详细比较。结果表明，等渗溶液中未变形的白色细胞为球形，具有许多膜折叠，并且具有比在血涂片上测量的直径显著更小的直径。采用化学固定的方法，使得由于细胞固定引起的收缩低于光学显微镜测量的分辨率。此外，研究表明，包括淋巴细胞在内的所有白细胞都具有比覆盖其体积所需的膜面积大得多的膜面积，并且当细胞低渗肿胀时，该膜面积保持恒定。
In order to establish quantitative models of leukocyte functions, several morphometric parameters on individual white cells are needed. These include the diameter, volume, and membrane area of the cells and their nuclei in the undeformed state. A stereologic method was used to obtain these quantities from transmission electron microscopy of random sections through human white blood cells (neutrophils, lymphocytes, monocytes, and eosinophils). In order to estimate possible artifacts due to preparation of the cells for transmission electron microscopy, a detailed comparison with light microscopy was made. The results show that undeformed white cells in isotonic solution are spherical with many membrane foldings and have a significantly smaller diameter than that measured on blood smears. A method of chemical fixation was employed so that the shrinkage due to fixation of the cells is below the resolution of light microscopic measurements. Further, it was shown that all leukocytes, including lymphocytes, have much more membrane area than is needed to cover their volumes, and this membrane area remains constant when the cell is hypotonically swollen.