Interfacing biodegradable molecular hydrogels with liquid crystals
Interfacing biodegradable molecular hydrogels with liquid crystals
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DOI：
10.1039/c2sm27160e
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发表时间：
2013-01-01
期刊：
影响因子：
3.4
通讯作者：
Webb, Simon J.
中科院分区：
文献类型：
--
作者：
Lin, I-Hsin;Birchall, Louise S.;Webb, Simon J.
自组装 Fmoc 肽水凝胶与液晶 (LC) 显示器连接，形成酶活性光学传感器。选择 Fmoc-TL-OMe 水凝胶是因为它可以通过酶介导的嗜热菌蛋白酶组装原位形成，并在添加枯草杆菌蛋白酶后进行酶介导的解组装。这种酶响应水凝胶提供了半刚性、高度水合和生物相容性的环境，也可将液晶显示器固定到位。开发了双层设计，其中加载磷脂的上凝胶层通过不含磷脂的下层与液晶显示器分开。枯草杆菌蛋白酶 (0.15 mM) 消化两层，几小时后产生凝胶到溶胶的转变，释放磷脂并在液晶显示器中产生由浅到深的光学变化。光学响应取决于凝胶到溶胶的转变；弹性蛋白酶或血清的常见成分不会分解 Fmoc-TL-OMe 水凝胶，也不会产生光学响应。
A self-assembled Fmoc-peptide hydrogel has been interfaced with a liquid crystal (LC) display to give an optical sensor for enzyme activity. An Fmoc-TL-OMe hydrogel was selected as it can be formed in situ by enzyme-mediated assembly with thermolysin, and undergoes enzyme-mediated diassembly upon subtilisin addition. This enzyme-responsive hydrogel provides a semi-rigid, highly hydrated and biocompatible environment that also holds the LC display in place. A dual layer design was developed, where a phospholipid-loaded upper gel layer was separated from the LC display by a phospholipid free lower layer. Subtilisin (0.15 mM) digested both layers to give a gel-to-sol transition after several hours that liberated the phospholipid and produced a light-to-dark optical change in the LC display. The optical response was dependent upon the gel-to-sol transition; elastase or common components of serum did not disassemble the Fmoc-TL-OMe hydrogel and did not give an optical response.