Highly specific and sensitive detection of bisphenol A in water samples using an enzyme-linked immunosorbent assay employing a novel synthetic antigen
Highly specific and sensitive detection of bisphenol A in water samples using an enzyme-linked immunosorbent assay employing a novel synthetic antigen
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DOI：
10.1039/c3nj01094e
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发表时间：
2014-01
影响因子：
3.3
通讯作者：
Wenbin Miao;Biwen Wei;Rongjing Yang;Chunhua Wu;D. Lou;Wei Jiang;Z. Zhou
中科院分区：
文献类型：
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作者：
Wenbin Miao;Biwen Wei;Rongjing Yang;Chunhua Wu;D. Lou;Wei Jiang;Z. Zhou
双酚A是一种新发现的环境中发现的具有雌激素性质的内分泌干扰物。随着塑料制品的广泛使用，双酚A在塑料制品中的扩散造成了严重的环境污染。本研究对双酚A抗原和多克隆抗体的合成进行了研究，以期建立一种快速检测水中双酚A的方法。在双酚A中引入了一个羧基，生成了一种能够与蛋白质偶联的新型半抗原。用合成的新抗原制备了具有良好特异性的兔多克隆抗体。所获得的抗体与其他类固醇类物质的交叉反应较低。建立的方法的半数抑制浓度为1.92±0.06μg L−1，最低检测限为0.1μg L−1，变异系数小于10%。水样的标准回收率为95.5%~105.0%。该方法简便、快速、专属性强，可用于水样中双酚A的定量检测。
Bisphenol A is a newly discovered endocrine disrupting chemical found in the environment that has estrogenic properties. With the extensive use of plastic products, the diffusion of bisphenol A from these products has caused serious environmental contamination. The present study investigated the synthesis of bisphenol-A antigen and polyclonal antibodies in order to develop a rapid method for the detection of bisphenol A in water. A carboxyl group was introduced into bisphenol A to create a novel hapten capable of coupling with proteins. A rabbit polyclonal antibody with good specificity was prepared by using the novel synthetic antigen. The obtained antibody had a low cross-reaction with other steroid substances. The half inhibitory concentration of the established method was 1.92 ± 0.06 μg L−1, with the lowest limit of detection being 0.10 μg L−1 and the coefficient of variation being less than 10%. The standard recovery rate of water samples was 95.5–105.0%. The method established in this study is an easy, rapid and specific method used for the quantitative detection of bisphenol A in water samples.