Functional anatomy of spontaneous seizures in a rat model of limbic epilepsy
Functional anatomy of spontaneous seizures in a rat model of limbic epilepsy
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DOI：
10.1111/j.1528-1157.1997.tb01083.x
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发表时间：
1997-01-01
期刊：
影响因子：
5.6
通讯作者：
Bertram, EH
中科院分区：
文献类型：
--
作者：
Bertram, EH
目的：部分性癫痫发作的发病机制尚不清楚。尽管许多研究暗示一个焦点将局限于一个单一的结构，但一些信息表明癫痫发作是区域性的，在几个不同的部位同时或几乎同时发作。为了确定癫痫发作的生理模式，在边缘癫痫大鼠模型上记录了多个边缘结构的自发性癫痫发作。方法：17只大鼠在电刺激海马诱发癫痫持续状态(SE)后发生慢性自发性边缘癫痫发作，在两侧中腹侧海马区和杏仁核或梨状皮质至少放置4个电极。根据癫痫发作的模式(局灶性海马区、局灶性非海马区或弥漫性)，对每只动物首次记录的15次自发性发作(平均12次发作，范围2-15次)进行评估。结果：总共评估了210次癫痫发作，从SE后1周开始，监测持续小于或等于18周(平均记录持续时间6周)，每只动物最多记录15次癫痫发作。总体而言，54%的癫痫发作为弥漫性发作，25%为非海马区发作，21%为海马区发作。17只动物中有8只(47%)有癫痫发作，所有三种发作模式都有。对动物从第一次癫痫发作到第十五次癫痫发作的模式演变的评估表明，较晚的癫痫发作有明显的弥漫性发作的趋势(回归分析r=0.737，p<；0.002)。结论：这些发现表明，在这种边缘“局灶性”癫痫模型中，致痫区域很宽，提示癫痫产生的底物分布在多个结构上。
Purpose: The substrate of seizure initiation in partial epilepsy is not well understood. Although many studies imply a focus that is will localized to a single structure, some information suggests that seizures arise regionally with simultaneous or near-simultaneous onset at several separate sites. To determine the physiological pattern of seizure onset, recordings were made of spontaneous seizures from multiple limbic structures in a rat model of limbic epilepsy.Methods: Seventeen rats with chronic spontaneous limbic seizures that occurred after an episode of electrical hippocampal stimulation-induced status epilepticus (SE) underwent chronic recording of seizures with a minimum of four electrodes placed bilaterally in the midventral hippocampus and either the amygdala or piriform cortex. As many as 15 of the first recorded spontaneous seizures (mean of 12 seizures, range 2-15) was evaluated for each animal with regard to pattern of seizure onset (focal hippocampal, focal nonhippocampal, or diffuse). The results were evaluated for number of seizure patterns displayed by each animal and the potential change in the pattern of onset with successive seizures (from the first to the fifteenth).Results: In all, 210 seizures were evaluated, beginning 1 week after SE and with monitoring lasting less than or equal to 18 weeks (mean recording duration 6 weeks) to record a maximum of 15 seizures for each animal. Overall, 54% of the seizures were of diffuse onset, 25% were of nonhippocampal onset, and 21% were of hippocampal onset. Eight of the 17 (47%) animals had seizures with all three patterns of onset. Evaluation of the evolution of the pattern of onset from the first to the fifteenth seizure recorded for the animals showed a clear tendency for the later seizures to have diffuse onsets (regression analysis r = 0.737, p < 0.002). Qualitative histologic analysis demonstrated neuronal loss in the recorded regions.Conclusions: These findings indicate that the epileptogenic zone is broad in this model of limbic ''focal'' epilepsy and suggest that the substrate for seizure generation is distributed over several structures.