Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker
Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker
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DOI：
10.1111/j.1365-2583.2006.00613.x
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发表时间：
2006-02-01
影响因子：
2.6
通讯作者：
Savakis, C
中科院分区：
文献类型：
--
作者：
Koukidou, M;Klinakis, A;Savakis, C
最近引入北美的橄榄果蝇（橄榄蝇）Bactrocera oleae (Dacus) 是世界范围内对橄榄最具破坏性的害虫。橄榄蝇缺乏有效的基因转移技术，阻碍了分子分析以及控制其遗传技术的发展。我们开发了一种基于 Minos 的转座子载体，其携带自激活盒，可过度表达增强型绿色荧光蛋白 (EGFP)。通过将该载体与体外合成的 Minos 转座酶 mRNA 共注射到前胚层胚胎中，在 B. oleae 种系中实现了该载体的一个到多个拷贝的高效转座酶介导整合。自激活基因构建体与转座酶 mRNA 相结合，形成了一个具有用于非常多样化物种转基因的潜力的系统。
The olive fruit fly (olive fly) Bactrocera oleae (Dacus), recently introduced in North America, is the most destructive pest of olives worldwide. The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos-based transposon vector carrying a self-activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase-mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. The self-activating gene construct combined with transposase mRNA present a system with potential for transgenesis of very diverse species.