Specific Induced Circular Dichroism and Enhanced B to Z Transitions of Duplexes Stabilized by Chromophore-Linked Alkynylnucleoside Residues
Specific Induced Circular Dichroism and Enhanced B to Z Transitions of Duplexes Stabilized by Chromophore-Linked Alkynylnucleoside Residues
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DOI：
10.1002/chem.200902882
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发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Inouye, Masahiko
中科院分区：
文献类型：
--
作者：
Fujimoto, Kazuhisa;Aizawa, Sayaka;Inouye, Masahiko
我们发展了一种带有发色团连接的炔基脱氧核糖骨架的诱导圆二色(ICD)探针，用于在可见光区域分析DNA双链的高阶结构。当5‘端带有探针的CG重复寡核苷酸(ODN)在高盐浓度下采用Z型双链时，在发色团的吸收区域观察到强烈的ICD信号。另一方面，它们在低盐浓度下形成的B-型双链产生微弱的ICD信号。发现只有当生色团连接在每个ODN的5‘端时，Z-型双链的特异性ICD才会出现。此外，发色的炔基核苷残基有效地促进了ODN的B-Z转变。
We have developed an induced circular dichroism (ICD) probe with a chromophore-linked alkynyldeoxyribose skeleton for analyzing higher-order structures of DNA duplexes in the visible-light region. When CG-repeated oligonucleotides (ODNs) with the probe at their 5' ends adopted Z-form duplexes at a high NaCl concentration, strong ICD signals were observed at the absorptive region of the chromophore. On the other hand, their B-form duplexes, formed at a low NaCl concentration, produced a faint ICD signal. The specific ICD for the Z-form duplexes was found to appear only when the chromophores were attached at the 5' ends of each of the ODNs. Furthermore, the chromophoric alkynylnucleoside residues effectively promoted the B to Z transition of the ODN.