Mapping of Powdery Mildew Resistance Gene pmCH89 in a Putative Wheat-Thinopyrum intermedium Introgression Line.
Mapping of Powdery Mildew Resistance Gene pmCH89 in a Putative Wheat-Thinopyrum intermedium Introgression Line.
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DOI：
10.3390/ijms160817231
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发表时间：
2015-07-28
影响因子：
5.6
通讯作者：
Chang Z
中科院分区：
文献类型：
--
作者：
Hou L;Zhang X;Li X;Jia J;Yang H;Zhan H;Qiao L;Guo H;Chang Z
白粉病，由 Blumeria graminis f 引起。 sp。小麦病害（Bgt）是一种对小麦生产产生不利影响的全球性严重病害。抗Bgt小麦育种系CH09W89是由Bgt抗性小麦-Thinopyrum intermedium部分双倍体TAI7045与感病小麦品种回交而得。在苗期，CH09W89表现出对Bgt致病型E09、E20、E21、E23、E26、Bg1和Bg2的免疫或高抗性，与其供体系TAI7045和Th相似。中间。没有Th。基于有丝分裂染色体的基因组原位杂交检测到中间染色质。为了确定Bgt抗性的遗传模式和抗性基因的染色体位置，将CH09W89与两个易感小麦品种杂交。遗传分析结果表明，CH09W89成虫对Bgt E09的抗性是由单个隐性基因控制的，暂定名为pmCH89。两个多态性SSR标记Xwmc310和Xwmc125与抗性基因连锁，遗传距离分别为3.1和2.7 cM。使用中国春非整倍体和缺失系，将抗性基因及其连锁标记分配到0.68-0.78 bin 中的染色体臂4BL。由于其在 4BL 染色体上的独特位置，pmCH89 似乎是抗白粉病的新基因座。这些结果将有利于提高小麦育种计划中的白粉病抗性。
Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally serious disease adversely affecting wheat production. The Bgt-resistant wheat breeding line CH09W89 was derived after backcrossing a Bgt resistant wheat-Thinopyrum intermedium partial amphiploid TAI7045 with susceptible wheat cultivars. At the seedling stage, CH09W89 exhibited immunity or high resistance to Bgt pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2, similar to its donor line TAI7045 and Th. intermedium. No Th. intermedium chromatin was detected based on genomic in situ hybridization of mitotic chromosomes. To determine the mode of inheritance of the Bgt resistance and the chromosomal location of the resistance gene, CH09W89 was crossed with two susceptible wheat cultivars. The results of the genetic analysis showed that the adult resistance to Bgt E09 in CH09W89 was controlled by a single recessive gene, which was tentatively designated as pmCH89. Two polymorphic SSR markers, Xwmc310 and Xwmc125, were linked to the resistance gene with genetic distances 3.1 and 2.7 cM, respectively. Using the Chinese Spring aneuploid and deletion lines, the resistance gene and its linked markers were assigned to chromosome arm 4BL in the bin 0.68–0.78. Due to its unique position on chromosome 4BL, pmCH89 appears to be a new locus for resistance to powdery mildew. These results will be of benefit for improving powdery mildew resistance in wheat breeding programs.