Methylation of p16 CpG islands correlated with metastasis and aggressiveness in papillary thyroid carcinoma
Methylation of p16 CpG islands correlated with metastasis and aggressiveness in papillary thyroid carcinoma
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DOI：
10.1016/j.jcma.2012.11.007
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发表时间：
2013-03-01
影响因子：
3
通讯作者：
Liu, Baoguo
中科院分区：
文献类型：
--
作者：
Wang, Peng;Pei, Renguang;Liu, Baoguo
背景：CpG 岛甲基化导致 p16 失活是人类癌症（包括甲状腺乳头状癌 (PTC)）中常见的早期事件。本研究旨在观察甲状腺乳头状癌（PTC）中p16基因的甲基化状态及其与临床参数的相关性。方法：采用甲基化特异性PCR（MSP）分析74例PTC和21例癌旁正常甲状腺组织中p16基因的甲基化状态。结果：27.0%（20/74）的PTC中p16基因高甲基化。与 PTC 相比，正常甲状腺组织均未甲基化 (p < 0.05)。 p16基因甲基化与患者的年龄、性别、肿瘤大小、组织学亚型和复发性疾病的发生无显着关系(p > 0.05)。 p16基因甲基化与转移、高AMES（年龄、远处转移、甲状腺外侵犯、大小）风险组（p < 0.05）和晚期病理肿瘤淋巴结转移分期呈正相关。结论：p16基因甲基化是分子生物学中具有重要意义的事件之一，在PTC中很常见，并且与生物转移和组织学特征相关，并且可能参与甲状腺肿瘤的发生和侵袭性。版权所有(C) 2012 Elsevier 台湾有限责任公司和中华医学会。版权所有。
Background: Inactivation of p16 by methylation of CpG islands is a frequent early event in human cancers, including papillary thyroid carcinoma (PTC). This study was to observe the methylation status of the p16 gene in papillary thyroid carcinoma (PTC) and its correlation with clinical parameters.Methods: Methylation-specific PCR (MSP) was used to analyze the methylation status of the p16 gene in 74 PTCs and 21 adjacent normal thyroid tissues.Results: Hypermethylation of p16 gene was observed in 27.0% (20/74) of PTC. None of the normal thyroid tissues was methylated, when compared to the PTCs (p < 0.05). There was no marked relationship between the methylation of p16 gene and the patients' age, gender, size of cancer, histological subtypes and occurrence of recurrent disease (p > 0.05). The methylation of p16 gene was positively associated with metastasis, a high AMES (age, metastasis to distant sites, extrathyroidal invasion, size) risk group (p < 0.05) and advanced pathological tumor lymph node-metastasis stages.Conclusion: The methylation of the p16 gene, one event of significance in molecular biology, was common and correlated with biological metastasis and histological features in PTC, and may be involved in thyroid tumorigenesis and aggressiveness. Copyright (C) 2012 Elsevier Taiwan LLC and the Chinese Medical Association. All rights reserved.