The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation
The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation
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DOI：
10.1016/s0925-4773(03)00004-2
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发表时间：
2003-04-01
影响因子：
2.6
通讯作者：
Heisenberg, CP
中科院分区：
文献类型：
--
作者：
Kilian, B;Mansukoski, H;Heisenberg, CP
Wnt基因在脊椎动物原肠胚形成过程中起着重要的调控作用。在斑马鱼中，slb/wnt11是趋同和伸展运动所必需的，而不是原肠形成过程中细胞命运的规范。为了确定其他Wnt基因是否与slb/wnt11在功能上相互作用，我们分析了ppt/wnt5在斑马鱼原肠胚形成过程中的作用。Ppt /wnt5是母系提供的，并在原肠胚的所有阶段合子表达。对ppt突变体胚胎的分析表明，ppt /Wnt5在原肠原胚后部调节细胞伸长和收敛伸展运动，而其在更前区域的功能与Slb/Wnt11相比在很大程度上是多余的。Frizzled-2在ppt/wnt5的下游发挥作用，说明在这一过程中它可能是ppt/wnt5的受体。Ppt/Wnt5在调节脊椎动物原肠胚运动中的作用的特征提供了对Wnt基因功能多样性的深入了解。(C) 2003爱思唯尔科学爱尔兰有限公司版权所有。
Wnt genes play important roles in regulating patterning and morphogenesis during vertebrate gastrulation. In zebrafish, slb/wnt11 is required for convergence and extension movements, but not cell fate specification during gastrulation. To determine if other Wnt genes functionally interact with slb/wnt11, we analysed the role of ppt/wnt5 during zebrafish gastrulation. ppt/wnt5 is maternally provided and zygotically expressed at all stages during gastrulation. The analysis of ppt mutant embryos reveals that Ppt/Wnt5 regulates cell elongation and convergent extension movements in posterior regions of the gastrula, while its function in more anterior regions is largely redundant to that of Slb/Wnt11. Frizzled-2 functions downstream of ppt/wnt5, indicating that it might act as a receptor for Ppt/Wnt5 in this process. The characterisation of the role of Ppt/Wnt5 provides insight into the functional diversity of Wnt genes in regulating vertebrate gastrulation movements. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.