Hormone-regulated inflorescence induction and TFL1 expression in Arabidopsis callus in vitro
Hormone-regulated inflorescence induction and TFL1 expression in Arabidopsis callus in vitro
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DOI：
10.1007/s00299-006-0165-y
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发表时间：
2006-11-01
影响因子：
6.2
通讯作者：
Zhang, X. S.
中科院分区：
文献类型：
--
作者：
Guan, C. M.;Zhu, S. S.;Zhang, X. S.
为研究拟南芥花序发育的调控机制，建立了细胞分裂素和生长素诱导的拟南芥花序离体再生体系。使用含有噻苯隆（TDZ）和2，4-二氯苯氧乙酸（2，4-D）的组合的培养基来诱导愈伤组织形成。以花序茎段为外植体，愈伤组织发生率较高。将愈伤组织转移到含有玉米素和吲哚-3-乙酸（IAA）的组合的培养基上后，从愈伤组织诱导花序。再生花序的形态与植物花序相似，但再生花序的花往往缺少一些花器官。此外，TFL 1，一个基因参与花的转变在拟南芥，在花序诱导过程中被激活。我们的研究结果表明，TFL 1基因起着重要的作用，在花调节花序的形成。
To study hormone-regulated inflorescence development, we established the in vitro regeneration system of Arabidopsis inflorescences in the presence of cytokinin and auxin. Media containing a combination of thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) were used to induce callus formation. Higher frequencies of calli were obtained by using the inflorescence stems as explants. After transferring the calli to media containing a combination of zeatin and indole-3-acetic acid (IAA), the inflorescences were induced from the calli. The morphology of regenerated inflorescences was similar to that of inflorescences in plants; however, flowers of regenerated inflorescences often lacked a few floral organs. Furthermore, TFL1, a gene involved in floral transition in Arabidopsis, was activated during the inflorescence induction. Our results suggest that the TFL1 gene plays an important role in hormone-regulated inflorescence formation.