Noncontact Nanoscale Imaging of Cells
Noncontact Nanoscale Imaging of Cells
复制标题
DOI：
10.1146/annurev-anchem-091420-120101
复制
发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Shevchuk, Andrew
中科院分区：
文献类型：
--
作者：
Klenerman, David;Korchev, Yuri;Shevchuk, Andrew
当细移液管接近细胞表面时，离子电流会减少，从而可以在不接触脆弱的细胞表面的情况下以纳米级分辨率对细胞表面形貌进行成像。已经开发和改进了多种不同的方法来以高分辨率和速度扫描动态细胞表面的形貌。细胞形貌的测量可以通过使用相同的移液器对细胞表面进行局部探测或绘图来补充。这可以通过执行单通道记录、施加力、输送激动剂、使用包含电化学探针的移液器或与荧光成像相结合来完成。这些方法相结合具有在纳米尺度上对活细胞表面进行成像和绘图的巨大潜力。
The reduction in ion current as a fine pipette approaches a cell surface allows the cell surface topography to be imaged, with nanoscale resolution, without contact with the delicate cell surface. A variety of different methods have been developed and refined to scan the topography of the dynamic cell surface at high resolution and speed. Measurement of cell topography can be complemented by performing local probing or mapping of the cell surface using the same pipette. This can be done by performing single-channel recording, applying force, delivering agonists, using pipettes fabricated to contain an electrochemical probe, or combining with fluorescence imaging. These methods in combination have great potential to image and map the surface of live cells at the nanoscale.