Lactate dehydrogenase in estrogen-responsive human breast cancer cells.
Lactate dehydrogenase in estrogen-responsive human breast cancer cells.
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DOI：
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发表时间：
1978-09
期刊：
影响因子：
11.2
通讯作者：
R. Burke;S. Harris;W. McGuire
中科院分区：
文献类型：
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作者：
R. Burke;S. Harris;W. McGuire
在来自密歇根癌症基金会的转移性乳腺癌患者的MCF-7人乳腺癌细胞系中测定乳酸脱氢酶活性（LDH）。在46，000 Xg细胞裂解物上清液中发现LDH，但在培养基中未发现LDH。只有第五种同工酶（LDH-5）可以通过醋酸纤维素电泳和相对热失活研究来证明。当内源性类固醇被从培养基中删除，除了雌激素的生长培养基几天升高LDH 2倍以上的控制; LDH没有改变时，MCF-7细胞用孕酮，氢化可的松，催乳素，胰岛素，或三碘甲状腺原氨酸处理。生理浓度（0.1 nM）的17 β-雌二醇足以产生最大LDH增加。雌激素对同工酶的影响不明显。因此，LDH活性可能是一个有用的标志物蛋白质研究激素的作用在MCF-7人乳腺癌细胞系。
Lactate dehydrogenase activity (LDH) was measured in the MCF-7 human breast cancer cell line derived at the Michigan Cancer Foundation from a patient with metastatic breast adenocarcinoma. LDH was found in the 46,000 X g supernatant of cell lysates, but not in the culture medium. Only the fifth isozyme (LDH-5) could be demonstrated by cellulose acetate electrophoresis and relative heat inactivation studies. When endogenous steroids were removed from the medium, addition of estrogen to the growth medium for several days elevated LDH 2-fold above controls; LDH was not altered when MCF-7 cells were treated with progesterone, hydrocortisone, prolactin, insulin, or triiodothyronine. A physiological concentration (0.1 nM) of 17beta-estradiol was sufficient to produce a maximal LDH increase. There were no qualitative isozyme changes in response to estrogen. LDH activity may therefore be a useful marker protein for studying hormone action in the MCF-7 human breast cancer cell line.