Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E-coli
Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E-coli
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DOI：
10.1016/j.febslet.2006.05.049
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发表时间：
2006-06-26
期刊：
影响因子：
3.5
通讯作者：
Gekko, Kunihiko
中科院分区：
文献类型：
--
作者：
Yamamoto, Tatsuya;Izumi, Shunsuke;Gekko, Kunihiko
大肠杆菌的70 S核糖体是一个超宏观复合体（MW：2.7 MDa），由3个RNA分子和50多种蛋白质组成。我们首次成功地分析了70 S核糖体蛋白质在溶液中的灵活性，通过检测氢/氘交换质谱。基于氘掺入图的X射线结构在每个交换的时间，我们展示了结构-柔性-功能的核糖体的关系，重点是氘掺入的蛋白质结合配体（tRNA，mRNA，和延伸因子）和结构组装过程的关系。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The 70S ribosome from Escherichia coli is a supermacro complex (MW: 2.7 MDa) comprising three RNA molecules and more than 50 proteins. We have for the first time successfully analyzed the flexibility of 70S ribosomal proteins in solution by detecting the hydrogert/deuterium exchange with mass spectrometry. Based on the deuterium incorporation map of the X-ray structure obtained at the time of each exchange, we demonstrate the structure-flexibility-function relationship of ribosome focusing on the deuterium incorporation of the proteins binding ligands (tRNA, mRNA, and elongation factor) and the relation with structural assembly processes. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.