Cellular micromotion monitored by long-range surface plasmon resonance with optical fluctuation analysis.
Cellular micromotion monitored by long-range surface plasmon resonance with optical fluctuation analysis.
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DOI：
10.1021/ac5031978
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发表时间：
2015-01
影响因子：
7.4
通讯作者：
Chih‐Tsung Yang;Régis Méjard;H. J. Griesser;P. Bagnaninchi;B. Thierry
中科院分区：
文献类型：
--
作者：
Chih‐Tsung Yang;Régis Méjard;H. J. Griesser;P. Bagnaninchi;B. Thierry
长程表面等离子体共振（LRSPR）是一种功能强大的生物传感技术，因为与传统SPR相比，它具有更大的探测深度和灵敏度。我们在这里证明，LRSPR可以提供敏感的非侵入性测量的动态波动的粘附细胞，通常被称为细胞的微动。使用3 T3成纤维细胞和MDA-MB-231癌细胞的汇合层实现概念验证。计算光波动的功率谱密度（PSD）的斜率以确定微动指数，并且测量活细胞层和固定细胞层之间的显著差异。此外，LRSPR和传统的表面等离子体共振（cSPR）的性能进行了比较方面的微动监测。我们的研究表明，LRSPR传感器测量的细胞微动指数高于cSPR测量时，表明LRSPR对细胞微动的灵敏度更高。为了进一步研究这一发现，进行模拟以建立LRSPR和cSPR对膜波动的相对灵敏度。与cSPR相比，预测LRSPR的信号强度增加，表明膜波动在LRSPR中测量的光学微动中起重要作用。类似于使用阻抗技术测量的细胞微动，LRSPR微动具有提供关于贴壁细胞的代谢活性和活力的重要生物信息的潜力。
Long-range surface plasmon resonance (LRSPR) is a powerful biosensing technology due to a substantially larger probing depth into the medium and sensitivity, compared with conventional SPR. We demonstrate here that LRSPR can provide sensitive noninvasive measurement of the dynamic fluctuation of adherent cells, often referred to as the cellular micromotion. Proof of concept was achieved using confluent layers of 3T3 fibroblast cells and MDA-MB-231 cancer cells. The slope of the power spectral density (PSD) of the optical fluctuations was calculated to determine the micromotion index, and significant differences were measured between live and fixed cell layers. Furthermore, the performances of LRSPR and conventional surface plasmon resonance (cSPR) were compared with respect to micromotion monitoring. Our study showed that the micromotion index of cells measured by LRSPR sensors was higher than when measured with cSPR, suggesting a higher sensitivity of LRSPR to the micromotion of cells. To investigate further this finding, simulations were conducted to establish the relative sensitivities of LRSPR and cSPR to membrane fluctuations. Increased signal intensity was predicted for LRSPR in comparison to cSPR, suggesting that membrane fluctuations play a significant role in the optical micromotion measured in LRSPR. Analogous to cellular micromotion measured using impedance techniques, LRSPR micromotion has the potential to provide important biological information on the metabolic activity and viability of adherent cells.