Two types of network oscillations in neocortex mediated by distinct glutamate receptor subtypes and neuronal populations
Two types of network oscillations in neocortex mediated by distinct glutamate receptor subtypes and neuronal populations
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DOI：
10.1152/jn.1996.75.2.951
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发表时间：
1996-02-01
影响因子：
2.5
通讯作者：
Connors, BW
中科院分区：
文献类型：
--
作者：
Flint, AC;Connors, BW
1.采用场电位记录方法，研究了两种不同形式的大鼠体感皮层自发同步振荡.第一类同步振荡由低胞外[Mg 2 +]激活，主频率为8-12 Hz。它被N-甲基-D-天冬氨酸（NMDA）受体拮抗剂D-2-氨基-5-膦酰基戊酸可逆地消除，而不受非NMDA受体拮抗剂6，7-二硝基喹喔啉-2，3-二酮（DNQX）的影响。用荷包牡丹碱阻断γ-氨基丁酸-A受体或用反式-1-氨基环戊烷-1，3-二羧酸激活代谢型谷氨酸受体可增加振荡事件的持续时间.第二种形式的同步振荡被激活急性应用红藻氨酸（10 μ M），有1-5赫兹的主频，并取消可逆DNQX。低浓度的软骨藻酸模拟红藻氨酸的作用，但α-氨基-3-羟基-5-甲基-4-异恶唑-丙酸或使君子酸没有，这表明GluR 5 -7和KA 1 -2谷氨酸受体亚基的作用.手术分离的皮质层表明，自发的NMDA受体依赖的振荡起源于层5内排他，但红藻氨酸受体依赖的振荡是唯一产生的神经元层2/3.5。我们的研究结果表明，新皮层神经元层2/3和层5可以独立地产生两种不同形式的节律性群体活动，每一个依赖于激活不同亚型的谷氨酸受体。
1. Two distinct forms of spontaneous synchronous oscillations were investigated with field potential recordings in slices of rat somatosensory cortex in vitro.2. The first type of synchronous oscillation was activated by low extracellular [Mg2+] and had dominant frequencies of 8-12 Hz. It was abolished reversibly by the N-methyl-D-aspartate (NMDA) receptor antagonist D-2-amino-5-phosphonovaleric acid and was relatively unaffected by the non NMDA receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX). The duration of oscillatory events was increased by blocking gamma-aminobuturic acid-A receptors with bicuculline or by activating metabotropic glutamate receptors with trans-1-aminocyclopentane-1,3-dicarboxylic acid.3. A second form of synchronous oscillation was activated by acute application of kainic acid (10 mu M), had dominant frequencies of 1-5 Hz, and was abolished reversibly by DNQX. Low concentrations of domoic acid mimicked the effects of kainate, but alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid or quisqualic acid did not, suggesting a role for the GluR5-7 and KA1-2 glutamate receptor subunits.4. Surgical isolation of cortical layers showed that spontaneous NMDA receptor-dependent oscillations originated within layer 5 exclusively, but kainate receptor-dependent oscillations were uniquely generated by neurons in layers 2/3.5. Our results suggest that neocortical neurons in layers 2/3 and layer 5 can independently generate two distinct forms of rhythmic population activity, each dependent upon activation of a different subtype of glutamate receptor.