Estimating cyanobacteria community dynamics and its relationship with environmental factors.
Estimating cyanobacteria community dynamics and its relationship with environmental factors.
复制标题
估计蓝藻群落动态及其与环境因素的关系
DOI：
10.3390/ijerph110101141
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发表时间：
2014-01-20
影响因子：
--
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Luo W;Chen H;Lei A;Lu J;Hu Z
采用传统显微计数法和PCR-DGGE基因分型法对铁岗水库和石岩水库富营养化水体中的蓝藻群落动态进行了研究。结果表明，蓝藻以纤细席藻（Phormidium tenue）为优势种，共鉴定出26种蓝藻，其中形态学鉴定14种，PCR-DGGE鉴定16种。蓝藻群落组成分析表明，从7月到12月的季节波动。铁岗水库和石岩水库的蓝藻数量在8月份达到高峰，细胞丰度分别为3.78 × 108 cells L-1和1.92 × 108 cells L-1。应用典范对应分析（CCA）进一步研究了蓝藻群落动态与环境因子的相关性。结果表明，蓝藻群落动态主要与pH、温度和总氮相关。本研究表明，PCR-DGGE结合传统形态学方法所获得的数据能够反映富营养化水体中蓝藻群落动态及其与环境因子的相关性。
The cyanobacteria community dynamics in two eutrophic freshwater bodies (Tiegang Reservoir and Shiyan Reservoir) was studied with both a traditional microscopic counting method and a PCR-DGGE genotyping method. Results showed that cyanobacterium Phormidium tenue was the predominant species; twenty-six cyanobacteria species were identified in water samples collected from the two reservoirs, among which fourteen were identified with the morphological method and sixteen with the PCR-DGGE method. The cyanobacteria community composition analysis showed a seasonal fluctuation from July to December. The cyanobacteria population peaked in August in both reservoirs, with cell abundances of 3.78 × 108 cells L-1 and 1.92 × 108 cells L-1 in the Tiegang and Shiyan reservoirs, respectively. Canonical Correspondence Analysis (CCA) was applied to further investigate the correlation between cyanobacteria community dynamics and environmental factors. The result indicated that the cyanobacteria community dynamics was mostly correlated with pH, temperature and total nitrogen. This study demonstrated that data obtained from PCR-DGGE combined with a traditional morphological method could reflect cyanobacteria community dynamics and its correlation with environmental factors in eutrophic freshwater bodies.
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影响因子：
2.1
作者：
Otsuka, S;Suda, S;Watanabe, MM
通讯作者：
Watanabe, MM
影响因子：
2.7
作者：
Shapiro, J
通讯作者：
Shapiro, J
DOI：
10.1007/bf00038688
发表时间：
1987-04-30
期刊：
VEGETATIO
影响因子：
--
作者：
TER BRAAK, CJF
通讯作者：
TER BRAAK, CJF
影响因子：
4.4
作者：
Janse, I;Meima, M;Zwart, G
通讯作者：
Zwart, G
影响因子：
4.4
作者：
Wawer, C;Jetten, MSM;Muyzer, G
通讯作者：
Muyzer, G