P53 IS FREQUENTLY MUTATED IN BURKITTS-LYMPHOMA CELL-LINES
P53 IS FREQUENTLY MUTATED IN BURKITTS-LYMPHOMA CELL-LINES
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DOI：
10.1002/j.1460-2075.1991.tb07837.x
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发表时间：
1991-10-01
期刊：
影响因子：
11.4
通讯作者：
CROOK, T
中科院分区：
文献类型：
--
作者：
FARRELL, PJ;ALLAN, GJ;CROOK, T
对12个Burkitt淋巴瘤细胞系和其他4个B细胞系进行了P53突变检测。使用突变特异性抗体进行的蛋白质分析以及对cDNA和基因组DNA的测序显示，在所研究的16个品系中，有12个品系相对于标准的p53蛋白序列发生了变化，其中包括10个BL品系。在BL系中，P53基因突变通常伴随着另一个等位基因的丢失。对突变的P53的cDNA进行转化活性的获得或生长抑制活性的丧失的测试表明，几个BL突变体在功能上与野生型P53发生了改变。
A panel of 12 Burkitt's lymphoma cell lines and four other B cell lines were tested for the presence of mutations in p53. Protein analysis using a mutant-specific antibody and sequencing of both cDNA and genomic DNA revealed changes relative to the standard p53 protein sequence in 12 of the 16 lines studied, including 10 of the BL lines. Mutation of p53 in the BL lines was usually accompanied by loss of the other allele of p53. Testing of the mutated p53 cDNAs for gain of transforming activity or loss of growth suppression activity showed that several of the BL mutants were functionally altered from wild-type p53.