CELLULAR AND ULTRASTRUCTURAL LOCATION OF ANGIOTENSINOGEN IN RAT AND SHEEP KIDNEY
CELLULAR AND ULTRASTRUCTURAL LOCATION OF ANGIOTENSINOGEN IN RAT AND SHEEP KIDNEY
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DOI：
10.1038/ki.1994.445
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发表时间：
1994-12-01
影响因子：
19.6
通讯作者：
COGHLAN, JP
中科院分区：
文献类型：
--
作者：
DARBY, IA;CONGIU, M;COGHLAN, JP
血管紧张素原在大鼠和绵羊肾脏的细胞定位和超微结构定位。最近的证据表明，局部的肾素-血管紧张素系统参与了血管紧张素II（Ang II）的某些肾脏作用。在这项研究中，肾脏分布的前体血管紧张素形成，血管紧张素原，在大鼠和绵羊进行了研究，采用免疫组织化学，免疫电镜和非同位素杂交组织化学。在大鼠和绵羊肾脏的近端小管（PCT）中观察到血管紧张素原的免疫染色。在大鼠中，新生（1天）大鼠肾脏中的免疫染色最强。出生后染色下降。使用生物素标记的寡脱氧核苷酸探针进行非同位素杂交组织化学证实了大鼠肾皮质PCT中存在血管紧张素原mRNA表达。电子显微镜免疫组化使用的抗体对大鼠血管紧张素原显示弱染色的颗粒状结构接近PCT细胞的顶膜成人。在新生大鼠肾脏，血管紧张素原免疫染色被发现在整个PCT细胞，并明显强于成年大鼠肾脏中看到的。在绵羊中，血管紧张素原免疫染色与纯化的羊血管紧张素原的抗体提出了PCT在胎儿，新生儿和成年羊肾脏染色。最强的免疫染色见于胎羊肾，出生后免疫染色下降。逆转录聚合酶链反应（RT-PCR）结果表明，血管紧张素原mRNA在绵羊肾脏中的表达在所有年龄的研究。血管紧张素原在胎羊肾（妊娠77天和141天）中的表达高于成年羊肾。总之，血管紧张素原mRNA的表达检测在大鼠和绵羊肾脏。免疫组化显示肾皮质PCT细胞内有血管紧张素原蛋白表达。血管紧张素原染色和mRNA表达在发育过程中最高，在成年后下降。
Cellular and ultrastructural location of angiotensinogen in rat and sheep kidney. Recent evidence suggests the involvement of a local renin-angiotensin system in some renal actions of angiotensin II (Ang II). In this study the renal distribution of the precursor to angiotensin formation, angiotensinogen, was investigated in rats and sheep using immunohistochemistry, immunoelectron microscopy and non-isotopic hybridization histochemistry. Immunostaining for angiotensinogen was seen in proximal tubules (PCT) of both rat and sheep kidneys. In the rat the strongest immunostaining was found in the kidneys of neonatal (1 day old) rats. Staining declined after birth. Non-isotopic hybridization histochemistry using oligodeoxynucleotide probes labeled with biotin confirmed the presence of angiotensinogen mRNA expression in PCT of the rat renal cortex. Electron microscopic immunohistochemistry using antibodies raised against rat angiotensinogen showed weak staining in the adult of granule-like structures close to the apical membrane of PCT cells. In the neonatal rat kidney, angiotensinogen immunostaining was found throughout the PCT cells and was markedly stronger than that seen in adult rat kidney. In sheep, angiotensinogen immunostaining with an antibody raised against purified ovine angiotensinogen showed staining of PCT in fetal, newborn and adult sheep kidney. The strongest immunostaining seen was in fetal sheep kidney with a decline seen after birth. Reverse transcription polymerase chain reaction (RT-PCR) showed that angiotensinogen mRNA was expressed in the sheep kidney at all ages studied. Angiotensinogen expression was higher in fetal sheep kidneys (77 day and 141 day gestation) than in adult sheep kidney. In conclusion, angiotensinogen mRNA expression was detected in both rat and sheep kidneys. Immunostaining showed angiotensinogen protein in PCT cells of the renal cortex. Angiotensinogen staining and mRNA expression is highest during development and declines in the adult.