Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage
Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage
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DOI：
10.1006/bbrc.1997.6622
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发表时间：
1997-05-08
影响因子：
3.1
通讯作者：
Katakura, Y
中科院分区：
文献类型：
--
作者：
Shirahata, S;Kabayama, S;Katakura, Y
活性氧或自由基对生物大分子造成广泛的氧化损伤，导致多种疾病和衰老。理想的活性氧清除剂应该是“活性氢”。在电解水的过程中，在阴极附近的还原水中可以产生“活性氢”。还原水表现出高pH、低溶解氧（DO）、极高溶解分子氢（DH）和极负的氧化还原电位（RP）值。强电解还原水以及抗坏血酸、（+）-儿茶素和单宁酸能完全清除O-2（.-）由次黄嘌呤-黄嘌呤氧化酶（HX-XOD）系统在磷酸钠缓冲液（pH 7.0）中产生。还原水的超氧化物歧化酶（SOD）样活性在4 ℃下稳定超过一个月，并且即使在中和、反复冷冻和熔化、超声处理放气、剧烈混合、煮沸、反复过滤或密闭高压灭菌后也没有损失，但是在有效吸附活性原子氢的三氧化钨的存在下通过开放高压灭菌或密闭高压灭菌而损失。用氢气鼓泡的水表现出低DO、极高DH和极低RP值，还原水也是如此，但它没有SOD样活性。这些结果表明，还原水的SOD样活性不是由于溶解的分子氢，而是由于溶解的原子氢（活性氢）。虽然SOD积累H_2O_2时，加入到HX-XOD系统，减少水减少了H_2O_2产生的XOD的量。还原水、过氧化氢酶和抗坏血酸都可以直接还原H2 O2。还原水以剂量依赖的方式抑制Cu（II）催化抗坏血酸氧化产生的活性氧对DNA单链的断裂，表明还原水不仅可以还原O-2（.和H_2O_2，还有O-1（2）和（OH）-O-。(C)北京：科学出版社.
Active oxygen species or free radicals are considered to cause extensive oxidative damage to biological macromolecules, which brings about a variety of diseases as well as aging. The ideal scavenger for active oxygen should be 'active hydrogen'. 'Active hydrogen' can be produced in reduced water near the cathode during electrolysis of water. Reduced water exhibits high pH, low dissolved oxygen (DO), extremely high dissolved molecular hydrogen (DH), and extremely negative redox potential (RP) values. Strongly electrolyzed-reduced water, as well as ascorbic acid, (+)-catechin and tannic acid, completely scavenged O-2(.-) produced by the hypoxanthine-xanthine oxidase (HX-XOD) system in sodium phosphate buffer (pH 7.0). The superoxide dismutase (SOD)-like activity of reduced water is stable at 4 degrees C for over a month and was not lost even after neutralization, repeated freezing and melting, deflation with sonication, vigorous mixing, boiling, repeated filtration, or closed autoclaving, but was lost by opened autoclaving or by closed autoclaving in the presence of tungsten trioxide which efficiently adsorbs active atomic hydrogen. Water bubbled with hydrogen gas exhibited low DO, extremely high DH and extremely low RP values, as does reduced water, but it has no SOD-like activity. These results suggest that the SOD-like activity of reduced water is not due to the dissolved molecular hydrogen but due to the dissolved atomic hydrogen (active hydrogen). Although SOD accumulated H2O2 when added to the HX-XOD system, reduced water decreased the amount of H2O2 produced by XOD. Reduced water, as well as catalase and ascorbic acid, could directly scavenge H2O2 Reduced water suppresses single-strand breakage of DNA by active oxygen species produced by the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner, suggesting that reduced water can scavenge not only O-2(.-) and H2O2, but also O-1(2) and (OH)-O-.. (C) 1997 Academic Press.