Copigmentation gives bluer flowers on transgenic torenia plants with the antisense dihydroflavonol-4-reductase gene
Copigmentation gives bluer flowers on transgenic torenia plants with the antisense dihydroflavonol-4-reductase gene
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DOI：
10.1016/s0168-9452(00)00364-2
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发表时间：
2000-12-07
期刊：
影响因子：
5.2
通讯作者：
Shibata, M
中科院分区：
文献类型：
--
作者：
Aida, R;Yoshida, K;Shibata, M
当植物中的花青素与辅色素（如黄酮或黄酮醇）形成复合物（辅色素）时，花朵的可见光吸收最大值发生变化，使其变得更长：也就是说，花朵看起来更蓝。在早期的一项研究中，我们的小组报告了在蓝猪耳（蓝猪耳Fournieri Lind.）通过重新引入二氢黄酮醇-4-还原酶（DFR）基因或查耳酮合酶（CNS）基因。我们对蓝猪耳转化体的初步观察是，具有反义DFR基因的植物比具有反义CHS基因的植物产生更蓝的花。在本研究中，我们发现，失活的DFR基因的遗传转化引起的黄酮类化合物的积累-可能的copigments -和由此产生的copigmentation可能使蓝猪耳花更蓝。这种方法可以应用于其他植物品种，以产生更蓝的花朵。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
When anthocyanins in plants make complexes with copigments such as flavones or flavonols (copigmentation), the visible absorption maximum of the flowers is shifted so that it becomes longer: that is, the flowers look bluer. In an earlier study, our group reported the modification of flower color in torenia (Torenia fournieri Lind.) by re-introduction of the dihydroflavonol-4-reductase (DFR) gene or the chalcone synthase (CNS) gene. Our initial observation of torenia transformants was that plants with the antisense DFR gene produced bluer flowers than plants with the antisense CHS gene. In the present study we found that inactivation of the DFR gene by genetic transformation caused the accumulation of flavones - possible copigments - and that the resulting copigmentation likely to make the torenia flowers bluer. This method could be applied to other plant species to produce bluer flowers. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.