Towards applications of synthetic genetic polymers in diagnosis and therapy.
Towards applications of synthetic genetic polymers in diagnosis and therapy.
复制标题
DOI：
10.1016/j.cbpa.2014.09.022
复制
发表时间：
2014-10
影响因子：
7.8
通讯作者：
Alexander I. Taylor;Sebastian Arangundy-Franklin;P. Holliger
中科院分区：
文献类型：
--
作者：
Alexander I. Taylor;Sebastian Arangundy-Franklin;P. Holliger
摘要核酸适体是一种具有高亲和力和特异性的核酸配体，近年来的发展增强了其在诊断和治疗中的应用前景。我们研究了具有潜在应用的核酸化学复制。我们总结了最近改进的核酸适体选择方法。核酸适体是一类单链核酸配体，它可以以高特异性和与抗体相媲美的亲和力结合其靶点。20多年前，Tuerk & Gold [1]和Ellington & Szostak [2]（发明了这个名字）首次描述了它们，它们作为诊断和治疗药物的前景仍有待实现。关键问题包括标准DNA/RNA或混合RNA/2′ F-DNA骨架在生理条件下的生物稳定性普遍较低，天然核碱基上官能团的化学多样性有限，本文综述了近年来在开发适体筛选技术以及扩展适体化学和信息复杂性以提高适体分子的生物学活性方面取得的进展。发现和属性。
HighlightsAptamers are nucleic acid ligands capable of high affinity and specificity.Recent developments enhance prospects for application in diagnosis and therapy.We examine replication of nucleic acid chemistries with potential applications.We summarise recent refinements of aptamer selection methodology.Aptamers are a class of single-stranded nucleic acid ligands that can bind their targets with high specificity and affinities rivalling those of antibodies. First described over 20 years ago by Tuerk & Gold [1] and Ellington & Szostak [2](who coined the name), their promise as both diagnostic and therapeutic agents remains to be realised. Key problems include the generally low biostability of the standard DNA/RNA or mixed RNA/2′ F-DNA backbones under physiological conditions, limited chemical diversity of functional groups on the natural nucleobases, and the difficulty in reliably discovering aptamer ligands to some therapeutic targets.This review will describe recent progress in developing aptamer selection technology as well as expanding aptamer chemistry and informational complexity to improve aptamer discovery and properties.