Mapping regulatory factors by immunoprecipitation from native chromatin.
Mapping regulatory factors by immunoprecipitation from native chromatin.
复制标题
通过天然染色质的免疫沉淀来绘制调节因子。
DOI：
10.1002/0471142727.mb2131s110
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发表时间：
2015
影响因子：
--
通讯作者：
Ahmad,Kami
中科院分区：
文献类型：
--
作者：
Orsi,GuillermoA;Kasinathan,Sivakanthan;Zentner,GabrielE;Henikoff,Steven;Ahmad,Kami
Occupied Regions of Genomes from Affinity‐purified Naturally Isolated Chromatin (ORGANIC) is a high‐resolution method that can be used to quantitatively map protein‐DNA interactions with high specificity and sensitivity. This method uses micrococcal nuclease (MNase) digestion of chromatin and low‐salt solubilization to preserve protein‐DNA complexes, followed by immunoprecipitation and paired‐end sequencing for genome‐wide mapping of binding sites. In this unit, we describe methods for isolation of nuclei and MNase digestion of unfixed chromatin, immunoprecipitation of protein‐DNA complexes, and high‐throughput sequencing to map sites of bound factors. © 2015 by John Wiley & Sons, Inc.
