Counterstaining to Separate Nile Red-Stained Microplastic Particles from Terrestrial Invertebrate Biomass
Counterstaining to Separate Nile Red-Stained Microplastic Particles from Terrestrial Invertebrate Biomass
复制标题
DOI：
10.1021/acs.est.0c00711
复制
发表时间：
2020-05-05
影响因子：
11.4
通讯作者：
Matthew, Grieshop
中科院分区：
文献类型：
--
作者：
Maxwell, Helmberger S.;Melinda, Frame K.;Matthew, Grieshop
微型塑料污染对土壤及其生物群构成的新威胁要求开发检测土壤动物摄取微型塑料的方法。尼罗红染料的荧光染色已被证明是区分微塑料与无机和某些生物材料的有效方法，但不适用于将它们与无脊椎动物遗骸分离。在这里，我们报告了一种新的荧光反染色技术的开发和验证，用于检测陆地无脊椎动物生物量和排泄物中的微塑料。在尼罗红的基础上，再用硫系荧光白和伊文思蓝的混合染料染色后，地面节肢动物的生物量呈现蓝紫色，而不同的塑料聚合物在激光扫描共聚焦显微镜下显示为红色、绿色和黄色。非节肢动物无脊椎动物的生物量和粪便材料也与塑料区分开来，尽管程度较小。我们的结果突出了这种方法在检测陆地无脊椎动物摄取微塑料方面的价值。
The emerging threat that microplastic pollution poses to soil and its biota necessitates the development of methods to detect microplastic ingestion by soil animals. Fluorescent staining with Nile red dye has proven to be effective at distinguishing microplastics from inorganic and some biological material but is not suitable for separating them from invertebrate remains. Here, we report on the development and validation of a novel fluorescent counterstaining technique for detection of microplastics within terrestrial invertebrate biomass and fecal material. After being stained with a blend of Calcofluor white and Evans blue dyes in addition to Nile red, ground arthropod biomass appeared blueish-purple, whereas different plastic polymers appeared red, green, and yellow when viewed under laser scanning confocal microscopy. Nonarthropod invertebrate biomass and fecal material were also distinguishable from plastic, though to a lesser extent. Our results highlight the value of this method for detecting microplastic ingestion by terrestrial invertebrates.