Quantitative and predictive model of transcriptional control of the Drosophila melanogaster even skipped gene
Quantitative and predictive model of transcriptional control of the Drosophila melanogaster even skipped gene
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DOI：
10.1038/ng1886
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发表时间：
2006-10-01
期刊：
影响因子：
30.8
通讯作者：
Reinitz, John
中科院分区：
文献类型：
--
作者：
Janssens, Hilde;Hou, Shuling;Reinitz, John
在这里，我们提出了果蝇基因控制区近端 1.7 kb 转录读出的定量和预测模型，甚至跳过了 (eve)。该模型基于 DNA 上各个结合位点的位置和序列以及细胞分辨率下的定量、时间分辨表达数据。这些数据展示了新的表达特征，首先在此报道。该模型正确预测了反式突变以及顺式点突变、插入和缺失的表达模式。它还表明，由该片段驱动的条带 7 的非经典表达被蛋白质 Caudal (Cad) 激活，并被蛋白质 Tailless (TII) 和 Giant (Gt) 抑制。
Here we present a quantitative and predictive model of the transcriptional readout of the proximal 1.7 kb of the control region of the Drosophila melanogaster gene even skipped ( eve). The model is based on the positions and sequence of individual binding sites on the DNA and quantitative, time-resolved expression data at cellular resolution. These data demonstrated new expression features, first reported here. The model correctly predicts the expression patterns of mutations in trans, as well as point mutations, insertions and deletions in cis. It also shows that the nonclassical expression of stripe 7 driven by this fragment is activated by the protein Caudal (Cad), and repressed by the proteins Tailless (TII) and Giant (Gt).