Complete genome sequence and molecular characterization of thermostable Newcastle disease virus strain TS09-C
Complete genome sequence and molecular characterization of thermostable Newcastle disease virus strain TS09-C
复制标题
耐热新城疫病毒株TS09-C的完整基因组序列和分子特征
DOI：
10.1007/s11262-012-0871-1
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发表时间：
2013-06-01
期刊：
影响因子：
1.6
通讯作者：
Cheng, Guofu
中科院分区：
文献类型：
--
作者：
Wen, Guoyuan;Shang, Yu;Cheng, Guofu
通过V4毒株在BHK-21细胞内的连续传代，获得了一株耐热无毒新城疫病毒（NDV） TS09-C。测定了菌株TS09-C的全基因组序列，并与其他具有不同耐热表型的NDV分离株的序列进行了比较。TS09-C基因组全长15186个核苷酸，由8个基因组成，序列为3′-NP-P/V/W-M-F-HN-L-5′。耐高温的NDV分离株具有较高的核苷酸和氨基酸序列一致性。耐热菌株TS09-C、V4、I-2和Ulster均属于基因型i。菌株TS09-C的F蛋白切割位点为112g - k - q - r - r - l117，分离出一个碱基氨基酸和一对相邻的碱基氨基酸，与亲本菌株V4 （112G-K-Q-G-R-L117）不同。本文对耐热新城疫无毒株TS09-C全基因组的鉴定，为建立耐热新城疫反遗传系统和进一步研究耐热新城疫的热稳定性机制提供了依据。
A thermostable avirulent Newcastle disease virus (NDV) strain TS09-C was developed by serial passage of V4 strain in BHK-21 cells. The complete genome sequence of strain TS09-C was determined and compared with the sequences of other NDV isolates representing different thermostable phenotypes. The TS09-C genome was 15,186 nucleotides long and consisted of eight genes in the order of 3′-NP-P/V/W-M-F-HN-L-5′. High levels of nucleotide and amino acid sequence identity existed among the thermostable NDV isolates. Thermostable strains TS09-C, V4, I-2, and Ulster all belonged to genotype I. The F protein cleavage site of strain TS09-C was112G-K-Q-R–R-L117, with an isolated basic amino acid and a pair of contiguous basic amino acids, differing from that of its parental V4 strain (112G-K-Q-G-R-L117). Our characterization of the complete genome of thermostable avirulent NDV strain TS09-C may facilitate the development of a thermostable NDV reverse genetic system and further study of the mechanism of thermostability of NDV.