First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype
First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype
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DOI：
10.1016/j.cmi.2016.07.032
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发表时间：
2016-11-01
影响因子：
14.2
通讯作者：
Maylin, S.
中科院分区：
文献类型：
--
作者：
Salmona, M.;Caporossi, A.;Maylin, S.
我们报告了来自刚果民主共和国的一名男子的丙型肝炎病毒（HCV）分离物的近全长基因组序列，该病毒的基因型无法通过常规测序技术确定。该变体BAK1的近完整基因组序列是通过两种下一代测序技术的联合获得的。进化分析表明，该分离物BAK1可能是首次报道的属于新型HCV-7b亚型的毒株。这种新的亚型被Versant HCV基因型2.0试验（LiPA）错误地鉴定为基因2型。三个独立分离株的需求已经满足，可以分配一个新的亚型。为了更好地了解HCV-7的起源、致病性及其与基因2型的关系，需要更多的HCV-7样本。M. Salmona, CMI 2016；22日:947. e1 - 947。e8 (C) 2016欧洲临床微生物学与传染病学会。Elsevier Ltd.出版。版权所有。
We report the near-full-length genome sequence of a hepatitis C virus (HCV) isolate from a man originating from Democratic Republic of Congo, the genotype of which could not be determined by the routinely used sequencing technique. The near-complete genome sequence of this variant BAK1 was obtained by the association of two next-generation sequencing technologies. Evolutionary analysis indicates that this isolate, BAK1, could be the first reported strain belonging to a new HCV-7b subtype. This new subtype has been incorrectly identified as genotype 2 by the Versant HCV Genotype 2.0 assay (LiPA). The requirement of three independent isolates has been filled, and a new subtype can be assigned. More examples of HCV-7 are required to better understand its origin, its pathogenicity and its relationship with genotype 2. M. Salmona, CMI 2016; 22: 947.e1-947.e8 (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.