REPLICATION OF AN INCOMPLETE ALFALFA MOSAIC-VIRUS GENOME IN PLANTS TRANSFORMED WITH VIRAL REPLICASE GENES
REPLICATION OF AN INCOMPLETE ALFALFA MOSAIC-VIRUS GENOME IN PLANTS TRANSFORMED WITH VIRAL REPLICASE GENES
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DOI：
10.1016/0042-6822(91)90876-d
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发表时间：
1991-04-01
期刊：
影响因子：
3.7
通讯作者：
BOL, JF
中科院分区：
文献类型：
--
作者：
TASCHNER, PEM;VANDERKUYL, AC;BOL, JF
苜蓿花叶病毒（alfalfa mosaic virus，AIMV）的RNA 1和RNA 2分别编码蛋白质P1和P2，这两种蛋白质在病毒RNA复制过程中起着重要作用。用RNA 1（P1-植物）、RNA 2（P2-植物）或这两种cDNA的组合（P12-植物）的DNA拷贝转化烟草植物。所有转基因植物都易受完整AIMV基因组（RNA 1、2和3）的感染。用AIMV RNA的不完全混合物接种表明，P1-植物能够复制RNA 2和3，P2-植物能够复制RNA 1和3，P12-植物能够复制RNA 3。非转基因植物、P1-植物或P2-植物的感染起始需要接种物中存在AIMV外壳蛋白，但不需要外壳蛋白来起始用RNA 3感染P12-植物。用P12原生质体获得的结果支持这样的结论，即外壳蛋白在AIMV RNA 1和2的复制周期中起重要作用。
RNAs 1 and 2 of alfalfa mosaic virus (AIMV) encode proteins P1 and P2, respectively, both of which have a putative role in viral RNA replication. Tobacco plants were transformed with DNA copies of RNA1 (P1-plants), RNA2 (P2-plants) or a combination of these two cDNAs (P12-plants). All transgenic plants were susceptible to infection with the complete AIMV genome (RNAs 1, 2, and 3). Inoculation with incomplete mixtures of AIMV RNAs showed that the P1-plants were able to replicate RNAs 2 and 3, that the P2-plants were able to replicate RNAs 1 and 3, and that the P12-plants were able to replicate RNA3. Initiation of infection of nontransgenic plants, P1-plants, or P2-plants requires the presence of AIMV coat protein in the inoculum, but no coat protein was required to initiate infection of P12-plants with RNA3. Results obtained with P12-protoplasts supported the conclusion that coat protein plays an essential role in the replication cycle of AIMV RNAs 1 and 2.