Long-lasting stability of Vaccinia virus strains in murine feces: implications for virus circulation and environmental maintenance
Long-lasting stability of Vaccinia virus strains in murine feces: implications for virus circulation and environmental maintenance
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DOI：
10.1007/s00705-009-0470-1
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发表时间：
2009-09-01
影响因子：
2.7
通讯作者：
Kroon, Erna Geessien
中科院分区：
文献类型：
--
作者：
Abrahao, Jonatas S.;Trindade, Giliane de Souza;Kroon, Erna Geessien
痘苗病毒（VACV）与巴西的几起牛痘苗爆发有关，导致奶牛和人类出现皮疹。 VACV 在环境中传播的方式以及该病毒从野生动物传播到农场的方式都是未知的。啮齿类动物是假设的 VACV 宿主，而小鼠粪便已被确定为病毒脱落和传播的潜在来源。在这项工作中，我们分别通过滴定测定和 PCR 分析了暴露于环境温度和湿度的鼻内感染小鼠粪便中 VACV 感染颗粒和 DNA 的稳定性。结果显示，在环境暴露后 20 天（d.p.e.）仍可检测到 VACV 感染性颗粒，而病毒 DNA 直到 60 d.p.e. 才可检测到。在所有分析的痘苗病毒株中都可以检测到粪便病毒载量逐渐减少。这项工作表明了 VACV 在小鼠粪便中的长期稳定性，并强化了粪便物质可能代表啮齿类动物中传播病毒的潜在来源的观点。
Vaccinia virus (VACV) has been associated with several bovine vaccinia outbreaks in Brazil, causing exanthematic lesions in dairy cattle and humans. The way that VACV circulates in the environment is unknown, as is the way that this virus is transferred from wildlife to farms. Rodents are hypothetical VACV reservoirs, and murine feces has been identified as a potential source of viral shedding and transmission. In this work, we analyzed the stability of VACV infectious particles and DNA in feces from intranasally infected mice, exposed to environmental temperature and humidity, by titration assays and PCR, respectively. The results showed that VACV infectious particles were still detected at 20 days post-environmental-exposure (d.p.e.), while viral DNA was detected until 60 d.p.e. A gradual decrease in fecal viral load could be detected in all analyzed VACV strains. This work indicates long-lasting stability of VACV in murine feces and reinforces the idea that fecal matter may represent a potential source of circulating virus among rodents.