IDENTIFICATION OF THE NONCOLLAGENOUS PROTEINS OF BOVINE BONE BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS
IDENTIFICATION OF THE NONCOLLAGENOUS PROTEINS OF BOVINE BONE BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS
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DOI：
10.1007/bf02405335
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发表时间：
1984-01-01
影响因子：
4.2
通讯作者：
MANN, KG
中科院分区：
文献类型：
--
作者：
DELMAS, PD;TRACY, RP;MANN, KG
采用2种高分辨率凝胶电泳技术对牛骨的非胶原蛋白进行了表征。用0.5 M EDTA延长透析时间，从骨组织中提取蛋白质。在某些情况下，用盐酸胍进行预提取。还检查了牛血浆，以确定骨骼中的蛋白质可能也存在于血液中。在标准制备骨图上对主要的、可复制的斑点（160个）进行评分。它们包括大约40个单独的蛋白质组。还有更多的小景点，使目前的总数超过200个。在这些群体中，有15个没有出现在血浆地图上。用这种方法鉴定的骨蛋白包括肌动蛋白、骨玻璃蛋白和骨连接蛋白。其余的是未知的。骨玻璃蛋白以4种等电形式存在于牛骨中，pI[等电点]= 3.95 ~ 4.50。EDTA和胍HCl萃取物的电印迹分析未发现任何更高分子量的免疫活性物质。在骨骼中发现的血浆蛋白包括但不限于白蛋白、载脂蛋白A-I、IgG、IgM、转铁蛋白、α。-2- hs糖蛋白和Hb。盐酸胍加EDTA萃取显著提高了非血浆蛋白的产量，但似乎没有提取任何额外的骨蛋白。
The noncollagenous proteins of bovine bone were characterized using 2 high-resolution gel electrophoretic techniques. Proteins were extracted from bone tissue by extended dialysis against 0.5 M EDTA. In some cases, a preextraction was done in guanidine HCl. Bovine plasma was also examined to identify the proteins in bone that might also be present in blood. Major, reproducible spots (160) were scored on a standard preparation bone map. These comprise about 40 individual protein groups. There are many more minor spots present which puts the total number present over 200. Of these groups, 15 are not present on plasma maps. Bone proteins identified in this way include actin, bone Gla-protein and osteonectin. The remainder are unknown. Bone Gla-protein is present in bovine bone in 4 isoelectric forms, pI [isoelectric point] = 3.95-4.50. Electroblotting analysis of EDTA and guanidine HCl extracted material failed to reveal any higher MW immunologically reactive species. The plasma proteins found in bone include, but are not limited to albumin, apo A-I lipoprotein, IgG, IgM, transferrin, .alpha.-2-HS-glycoprotein and Hb. Extraction with guanidine HCl plus EDTA significantly enriches the yield for the nonplasma proteins but does not appear to extract any additional bone proteins.