CHARACTERIZATION OF A CORONAVIRUS .1. STRUCTURAL PROTEINS - EFFECTS OF PREPARATIVE CONDITIONS ON MIGRATION OF PROTEIN IN POLYACRYLAMIDE GELS
CHARACTERIZATION OF A CORONAVIRUS .1. STRUCTURAL PROTEINS - EFFECTS OF PREPARATIVE CONDITIONS ON MIGRATION OF PROTEIN IN POLYACRYLAMIDE GELS
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DOI：
10.1016/0042-6822(77)90488-3
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发表时间：
1977-01-01
期刊：
影响因子：
3.7
通讯作者：
STURMAN, LS
中科院分区：
文献类型：
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作者：
STURMAN, LS
冠状病毒A59具有4种大小类别的结构蛋白，通过SDS[十二烷基硫酸钠]-聚丙烯酰胺凝胶电泳（SDS-PAGE）测得的表观MW为23，000（GP 23）、50，000（VP 50）、90，000（GP 90）和180，000（GP 180）。VP 50是唯一的结构蛋白，其完全不受蛋白酶处理完整病毒粒子的影响。该物种是最高度标记的极性氨基酸，如谷氨酸和精氨酸，它可能是与病毒核衣壳。GP 90、GP 180和GP 23是膜相关蛋白。在病毒粒子的蛋白酶处理后，只有20%的GP 23分子被消化，而所有的GP 90和GP 180被去除。GP 90和GP 180似乎包括病毒包膜外表面上的大部分突出的特征性突起层。推测GP 23的主要部分位于脂质包膜内，免受蛋白酶消化。GP 23和蛋白酶抗性部分p*18在SDS-PAGE上表现出异常行为。加热至100 ℃后，在SDS中，这些多肽的电泳迁移率被改变，并产生了几种新的低迁移率形式。β-巯基乙醇和二硫苏糖醇夸大了加热的效果。
Coronavirus A59 possesses 4 size classes of structural proteins which have apparent MW measured by SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis (SDS-PAGE) of 23,000 (GP23), 50,000 (VP50), 90,000 (GP90) and 180,000 (GP180). VP50 is the only structural protein which is completely unaffected by protease treatment of intact virions. This species is the most highly labeled by polar amino acids such as glutamic acid and arginine, and it is probably associated with the viral nucleocapsid. GP90, GP180 and GP23 are membrane-associated proteins. After protease treatment of virions, only 20% of the GP23 molecule is digested, whereas all of the GP90 and GP180 are removed. GP90 and GP180 appear to comprise most of the prominent layer of characteristic projections on the external surface of the viral envelope. The major portion of GP23 is presumed to lie within the lipid envelope, protected from protease digestion. GP23 and the protease resistant portion, p*18, exhibit anomalous behavior on SDS-PAGE. After heating to 100.degree. C in SDS the electrophoretic mobility of these polypeptides is altered, and several new forms of lower mobility are produced. .beta.-Mercaptoethanol and dithiothreitol exaggerate the effects of heating.