Ultra high performance liquid chromatography with mass spectrometry for the rapid analysis and global characterization of multiple constituents from Zibu Piyin Recipe
Ultra high performance liquid chromatography with mass spectrometry for the rapid analysis and global characterization of multiple constituents from Zibu Piyin Recipe
复制标题
超高效液相色谱-质谱联用技术对子补脾饮方中多种成分的快速分析和整体表征
DOI：
10.1002/jssc.201500852
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发表时间：
2016-02-01
影响因子：
3.1
通讯作者：
Liu, Yanqiu
中科院分区：
文献类型：
--
作者：
Dong, Peipei;Zhang, Lin;Liu, Yanqiu
滋补脾阴方是我国临床上用于治疗记忆力减退的中药。然而，迄今为止，其化学成分尚未得到彻底研究。为了快速识别化学成分并了解与认知改善活性相关的化学特征，应用了超高效液相色谱与电喷雾电离四极杆飞行时间质谱联用。分析采用 Agilent 1290 超高效液相色谱系统，采用 Poroshell 120 EC-C18 色谱柱（3.0 x 150 mm，2.7 μm），采用梯度洗脱。采用优化后的方法，初步鉴定出滋补脾饮方中的155种化学成分。其中，负离子模式可检出112种成分，正离子模式可检出73种成分，两种模式均可检出30种化合物。经鉴定，其主要成分为红参、丹参、白芍、甘草、远志、陈皮、草叶、黄芪、莲子。这些结果表明，所建立的快速、稳健的方法将有助于识别中药处方的多种成分。该方法可为进一步研究滋补脾阴方体内药理机制以及改善认知功能的新药开发提供有用的化学信息。
Zibu Piyin Recipe is a traditional Chinese medicine that has been used for the clinical treatment of memory loss in China. However, the chemical components have not been thoroughly studied so far. To quickly identify the chemical components and understand the chemical profiles related to cognition improvement activity, ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry has been applied. The analysis was performed on an Agilent 1290 ultra-high performance liquid chromatography system with a Poroshell 120 EC-C18 column (3.0 x 150 mm, 2.7 mu m) using gradient elution. Using the optimized method, 155 chemical components in Zibu Piyin Recipe were tentatively identified. Among them, 112 components in negative ion mode, 73 constituents in positive ion mode, and 30 compounds in both modes could be detected. The major components were identified from Red Ginseng, Dan shen Root, White Paeony Root, liquorice root, Radix Polygalae, Tangerine Red Epicarp, Grassleaf Sweetflag Rhizome, Indian Buead, and lotus seed. These results suggest that the established rapid and robust method will be useful for identifying multiple constituents of traditional Chinese medicine prescriptions. This method could provide helpful chemical information for further in vivo pharmacological mechanism research of Zibu Piyin Recipe and new drug development for cognition improvement.