CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes
CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes
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DOI：
10.1095/biolreprod.106.053181
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
Marunouchi, Tohru
中科院分区：
文献类型：
--
作者：
Tokuda, Masutaka;Kadokawa, Yuzo;Marunouchi, Tohru
在哺乳动物睾丸中，精子发生是由属于未分化的A型精原细胞的干细胞亚群启动的。尽管未分化的A型精原细胞具有重要的生物学意义，但由于缺乏特异性的细胞表面标志物，对其行为和特性知之甚少。在这里，我们表明，CDH 1（以前称为E-钙粘蛋白）在小鼠睾丸未分化的A型精原细胞中特异性表达。组织学分析表明，CDH 1阳性细胞具有未分化A型精原细胞的所有特征。整体免疫组化显示，CDH 1阳性细胞主要由一个，两个，四个或八个细胞组成的集群。它们在给予小鼠细胞毒性剂白消安后存活，然后再生生精上皮。移植实验表明，只有CDH 1阳性细胞在受体睾丸中具有定殖活性。我们的数据清楚地表明，生精干细胞存在于未分化的A型精原细胞，表达CDH 1。
In the mammalian testis, spermatogenesis is initiated from a subset of stem cells belonging to undifferentiated type A spermatogonia. In spite of the biologic significance of undifferentiated type A spermatogonia, little is known about their behavior and properties because of a lack of specific cell surface markers. Here we show that CDH1 (previously known as E-cadherin) is expressed specifically in undifferentiated type A spermatogonia in the mouse testis. Histologic analysis showed that CDH1-positive cells had all the characteristics of undifferentiated type A spermatogonia. Whole-mount immunohistochemistry showed that CDH1-positive cells made clusters mainly comprising one, two, four, or eight cells. They survived after administration of the cytotoxic agent busulfan to mice, and then regenerated seminiferous epithelia. Transplantation experiments showed that only CDH1-positive cells had colonizing activity in the recipient testis. Our data clearly demonstrated that spermatogenic stem cells reside among undifferentiated type A spermatogonia, which express CDH1.