The R3H domain stabilizes poly(A)-specific ribonuclease by stabilizing the RRM domain
The R3H domain stabilizes poly(A)-specific ribonuclease by stabilizing the RRM domain
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R3H 结构域通过稳定 RRM 结构域来稳定聚腺苷酸特异性核糖核酸酶
DOI：
10.1016/j.bbrc.2007.06.139
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发表时间：
2007-09-07
影响因子：
3.1
通讯作者：
Yan, Yong-Bin
中科院分区：
文献类型：
--
作者：
Liu, Wei-Feng;Zhang, Ao;Yan, Yong-Bin
Poly(A) 特异性核糖核酸酶 (PARN) 是一种参与真核 mRNA 降解的关键酶，包含一个催化结构域和两个 RNA 结合结构域。在这里，我们发现底物结合至少需要一个 RNA 结合结构域，但 PARN 的催化不需要。 R3H 结构域的去除导致 PARN 稳定性急剧下降和聚集动力学状态的变化，而 RRM 结构域或两个 RNA 结合结构域的去除仅观察到较小的影响。因此，R3H 结构域可能通过充当 RRM 结构域的保护器或分子间伴侣来稳定 PARN。 (c) 2007 Elsevier Inc. 保留所有权利。
Poly(A)-specific ribonuclease (PARN), a key enzyme involved in eukaryotic mRNA decay, contains one catalytic domain and two RNA-binding domains. Here we found that at least one RNA-binding domain is required for the substrate binding, but not for the catalysis of PARN. The removal of the R3H domain led to a dramatic decrease in PARN stability and a change in the aggregation kinetic regime, while only minor effects were observed for the removal of the RRM domain or both RNA-binding domains. Thus the R3H domain might stabilize PARN by acting as a protector or intermolecular chaperone of the RRM domain. (c) 2007 Elsevier Inc. All rights reserved.