Get effective polyclonal antisera in one month
Get effective polyclonal antisera in one month
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DOI：
10.1038/sj.cr.7290122
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发表时间：
2002-06-01
期刊：
影响因子：
44.1
通讯作者：
Zhang, YL
中科院分区：
文献类型：
--
作者：
Hu, YX;Guo, JY;Zhang, YL
按照传统免疫程序，首次注射样品A（目标抗原+弗氏完全佐剂乳剂）免疫家兔后，每隔2-4周连续注射样品B（目标抗原+弗氏不完全佐剂乳剂）。一般情况下，3-4个月内注射4-5次样品B即可观察到高滴度的相应多克隆抗血清。该报告提出了一种经过简单修改的程序，能够在一个月内刺激抗血清形成，并达到足够的亲和力以满足蛋白质印迹或免疫组织化学分析的要求。只是在初次免疫注射后的第3天，对兔子再次注射了样品A。一个月内第一次注射B样品后即可获得高滴度的抗血清。该方法在原核或真核细胞表达的三种不同分子量（5.9 KD-55 KD）的不同重组抗原中产生了所需的结果。
According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general, high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4 months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis. It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.