Modifying gene expression programs by altering core promoter chromatin architecture
Modifying gene expression programs by altering core promoter chromatin architecture
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DOI：
10.1016/s0092-8674(02)00822-x
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发表时间：
2002-07-26
期刊：
影响因子：
64.5
通讯作者：
Thanos, D
中科院分区：
文献类型：
--
作者：
Lomvardas, S;Thanos, D
响应于病毒感染的IFN-β基因的转录激活需要增强体的组装，增强体指示染色质修饰剂/重塑剂和一般转录因子向启动子的募集程序。这个程序的高潮是核小体将核心启动子阻断到下游位置，这是转录激活的先决条件。我们表明，交付这个核小体到相同的下游位置，以创建一个可访问的IFN-β核心启动子之前增强子组装的结果在基因表达程序的时间模式和信号特异性的转录反应方面的重大变化。因此，基因表达程序的同一性是通过特定增强体和特定局部染色质结构之间的动态相互作用来实现和维持的。
Transcriptional activation of the IFN-beta gene in response to virus infection requires the assembly of an enhanceosome, which instructs a recruitment program of chromatin modifiers/remodelers and general transcription factors to the promoter. This program culminates with sliding of a nucleosome blocking the core promoter to a downstream position, a prerequisite for transcriptional activation. We show that delivery of this nucleosome to the same downstream position to create an accessible IFN-beta core promoter prior to enhanceosome assembly results in major changes in the gene expression program with regard to the temporal pattern and the signal specificity of the transcriptional response. Thus, the identity of a gene expression program is achieved and maintained by the dynamic interplay between specific enhanceosomes and specific local chromatin structure.