Evidence for Multiple Origins of Knockdown Resistance (kdr) in Spodoptera exigua (Hübna) (Lepidoptera: Noctuidae) From China
Evidence for Multiple Origins of Knockdown Resistance (kdr) in Spodoptera exigua (Hübna) (Lepidoptera: Noctuidae) From China
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DOI：
10.1093/jee/toac075
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发表时间：
2022-05
影响因子：
2.2
通讯作者：
Yayun Zuo;Zeyu Wang;Xuan Ren;Yakun Pei;A. Aioub;Zhaonong Hu
中科院分区：
文献类型：
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作者：
Yayun Zuo;Zeyu Wang;Xuan Ren;Yakun Pei;A. Aioub;Zhaonong Hu
摘要甜菜夜蛾（Spodoptera exigua, h<s:1> bna）是一种严重的农业害虫，对包括拟除虫菊酯在内的大多数农药具有抗性，是一种具有挑战性的害虫。这种抗性先前与S. exigua电压门控钠通道（VGSC）的低敲抗性（kdr）突变（L1014F）有关。为了更好地了解短叶丝瓜SeVGSC的kdr突变频率，确定短叶丝瓜kdr突变的进化起源，在中国收集了7个短叶丝瓜种群，并获得了每个个体的部分SeVGSC基因组序列。生物测定结果表明，7个种群对高效氯氰菊酯（0.05 mg/ cm2）的存活率为91.66% ~ 100%，表明7个种群均对高效氯氰菊酯产生了抗性。在中国范围内的田间群体中，SeVGSC的kdr突变频率（CTT ~ TTT）在60% ~ 89.6%之间。该编码位置的CTT到CAT的替换导致L1014H （kdr-H）突变仅在QP18群体中的一个个体中发现。基于SeVGSC等位基因的系统发育分析，结果表明，赤眼蜂种群中的kdr突变具有多重来源，这对田间拟除虫菊酯类杀虫剂的有效性具有重要影响。因此，建议限制拟除虫菊酯类杀虫剂的使用，并鼓励不同作用方式的杀虫剂轮作防治，以减轻抗性的发展。
Abstract The beet armyworm, Spodoptera exigua (Hübna) is a serious agricultural pest that is challenging to control due to resistance to most pesticides, including pyrethroids. This resistance has previously been linked to the knockdown resistance (kdr) mutation (L1014F) of the voltage-gated sodium channel (VGSC) in S. exigua. To better understand the frequencies of the kdr mutation of SeVGSC and identify the evolutionary origins of kdr mutation in S. exigua, seven populations of S. exigua were collected in China, and partial SeVGSC genomic sequences for each individual were acquired. The bioassays showed that the survival rates of seven populations of S. exigua larvae exposed to the discriminating dose of beta-cypermethrin (0.05 mg/ cm2) ranged from 91.66% to 100%, indicating that all seven populations had evolved resistance to beta-cypermethrin. The frequencies of kdr mutation (CTT to TTT) of SeVGSC of field populations ranged China were from 60% to 89.6%. The CTT to CAT substitution at this coding position resulting in the L1014H (kdr-H) mutation was found in only one individual from the QP18 population. Based on the phylogeny of SeVGSC alleles, it appeared that the kdr mutation in S. exigua populations had multiple origins, which has major consequences for pyrethroid effectiveness in the field. Thus, it is recommended to limit the use of pyrethroid and encourage rotation of insecticides with different modes of action for control of S. exigua to alleviate resistance development.