Influence of cytokines and growth factors on matrix metalloproteinase-2 production and invasion of human renal cancer
Influence of cytokines and growth factors on matrix metalloproteinase-2 production and invasion of human renal cancer
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DOI：
10.1007/s002400050020
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发表时间：
1998-02-01
影响因子：
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通讯作者：
Kamidono, S
中科院分区：
文献类型：
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作者：
Gohji, K;Nomi, M;Kamidono, S
本研究评价了细胞因子和生长因子对人肾细胞癌（HRCC）细胞系KG-2的基质金属蛋白酶-2（MMP-2，72-kDa IV型胶原酶，明胶酶A）和侵袭的影响。用细胞因子和生长因子处理细胞，检测其溶胶活性和体外侵袭能力。碱性成纤维细胞生长因子（bFGF）在0.1、1.0和10 ng/ml浓度下分别刺激KG-2细胞产生MMP-2，是未处理组的2.0、4.84和4.53倍。转化生长因子-β 1（TGF-β 1）在10 pg/ml和100 pg/ml的极低浓度下分别刺激KG-2细胞中的酶产生1.74倍和2.83倍。与此相反，干扰素-γ（IFN-γ）降低MMP-2生产KG-2细胞在10和100 U/ml的69%和41%的水平，在未处理组，分别。在这些浓度下，IFN-γ在KG-2细胞中不引起细胞停滞。此外，bFGF和TGF-β 1（低浓度）刺激KG-2细胞的体外侵袭，但IFN-γ降低侵袭活性，这与MMP-2水平密切相关。然而，用10 ng/ml bFGF、100 pg/ml TGF-β 1和100 U/ml IFN-γ处理的KG-2细胞的MMP-2 mRNA表达分别是未处理组的3.8、3.4和0.7倍。因此，MMP-2在HRCC中的产生受细胞因子和生长因子的影响，并且MMP-2在某些类型的HRCC的侵袭和转移中起重要作用。
This study evaluated the influence of cytokines and growth factors on the production of matrix metalloproteinase-2 (MMP-2, 72-kDa type IV collagenase, gelatinase A) and invasion of the human renal cell carcinoma (HRCC) cell line KG-2. The cells were treated with cytokines and growth factors, and the gelatiolytic activity and in vitro invasion were examined. Basic fibroblast growth factor (bFGF) stimulated MMP-2 production by KG-2 cells to 2.0-, 4.84- and 4.53-fold that of the untreated group at 0.1, 1.0 and 10 ng/ml, respectively. Transforming growth factor-beta 1 (TGF-beta 1) at very low concentrations of 10 pg/ml and 100 pg/ml stimulated enzyme production in KG-2 cells by 1.74- and 2.83-fold, respectively. In contrast, interferon-gamma (IFN-gamma) decreased MMP-2 production by KG-2 cells at 10 and 100 U/ml to 69% and 41% of the level in the untreated group, respectively. At those concentrations, IFN-gamma did not cause cytostasis in KG-2 cells. Moreover bFGF and TGF-beta 1 (low concentrations) stimulated in vitro invasion of KG-2 cells, but IFN-gamma decreased the invasive activity, which was well correlated with the levels of MMP-2. However, the expression of MMP-2 mRNA of KG-2 cells treated with 10 ng/ml bFGF, 100 pg/ml TGF-beta 1 and 100 U/ml IFN-gamma was shown to be 3.8-, 3.4- and 0.7-fold, respectively, those in untreated groups. Thus the production of MMP-2 in HRCC was influenced by cytokines and growth factors, and MMP-2 plays an important role in the invasion and metastasis of certain types of HRCC.