Photothermal-Induced Electrochemical Interfacial Region Regulation Enables Signal Amplification for Dual-Mode Detection of Ovarian Cancer Biomarkers
Photothermal-Induced Electrochemical Interfacial Region Regulation Enables Signal Amplification for Dual-Mode Detection of Ovarian Cancer Biomarkers
复制标题
光热诱导电化学界面区域调节可实现卵巢癌生物标志物双模式检测的信号放大
DOI：
10.1021/acsabm.1c00665
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发表时间：
2021
影响因子：
4.7
通讯作者：
Yanyu Lin
中科院分区：
文献类型：
--
作者：
Shupei Zhang;Huizhu Ren;Hong Dai;Liang Lv;Yanyu Lin
电化学免疫传感器的检测灵敏度主要取决于到传感界面的可及距离，调节其上的电化学界面区域是一种有效的信号放大策略。为此，设计了一种基于近红外(NIR)响应型水凝胶探针的光热调节传感接口，用于超灵敏地检测人附睾蛋白4(HE4)。将银纳米粒子沉积的氧化石墨烯纳米片(AgNPs@GO)作为电化学信号标签，将光热传感器包裹在聚N-异丙基丙烯酰胺(PNIPAM)水凝胶中，制备了近红外响应的GO@AgNPs-PNIPAM水凝胶探针。在近红外光照射下，AgNPs@GO杂化材料具有良好的光热效应，不仅可以迅速将近红外光转化为热用于温度读出，还可以引发水凝胶的收缩行为，用于电化学信号放大。与传统的夹心免疫分析相比，水凝胶信号探针的收缩行为赋予了其界面调节能力，从而提高了电化学反应效率；在保证扩展的外亥姆霍兹平面(OHP)区域的基础上，所提出的光热诱导的界面调节也缩短了OHP，从而导致更高的灵敏度。此外，所获得的双模信号为肿瘤标志物的检测提供了令人满意的准确性。该检测方案为光热效应在临床诊断中的广泛应用提供了机会。
Detection sensitivity of an electrochemical immunosensor mainly depends on the accessible distance toward the sensing interface; regulating the electrochemical interfacial region thereon is an effective strategy for signal amplification. Herein, a photothermal-regulated sensing interface was designed based on a near-infrared (NIR)-responsive hydrogel probe for ultrasensitive detection of human epididymis protein 4 (HE4). Silver nanoparticle-deposited graphene oxide nanosheet (AgNPs@GO) hybrids as electrochemical signal tags and a photothermal transducer, which were encapsulated in the poly(N-isopropylacrylamide) (pNIPAM) hydrogel, were used to develop the NIR-responsive GO@AgNPs-pNIPAM hydrogel probe. Under NIR light irradiation, the excellent photothermal effect of AgNPs@GO hybrids not only rapidly converted NIR light into heat for temperature readout but also triggered the shrinkage behavior of the hydrogel for electrochemical signal amplification. Compared with the conventional sandwich immunoassay, the shrinkage behavior of the hydrogel signal probe endowed itself with interface regulation capability to improve the electrochemical reaction efficiency; on the basis of ensuring the extended outer Helmholtz plane (OHP) region, the proposed photothermal-induced interface regulation also shortened the OHP, leading to higher sensitivity. Moreover, the obtained dual-mode signals provided satisfactory accuracy for the detection of tumor markers. Therefore, this detection scheme provided an opportunity for the broad applications of the photothermal effect in clinical diagnosis.