Position dependence of the Rous sarcoma virus negative regulator of splicing element reflects proximity to a 5' splice site.
Position dependence of the Rous sarcoma virus negative regulator of splicing element reflects proximity to a 5' splice site.
复制标题
劳斯肉瘤病毒剪接元件负调节因子的位置依赖性反映了与 5 剪接位点的接近性。
DOI：
10.1016/s0042-6822(03)00378-7
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发表时间：
2003
期刊：
影响因子：
3.7
通讯作者：
McNally,MarkT
中科院分区：
文献类型：
--
作者：
Wang,Yuedi;McNally,MarkT
Rous肉瘤病毒(RSV)需要其病毒转录本的不完全剪接来维持有效的复制。剪接抑制元件是剪接的负调控元件，位于RNA的5‘端附近，但这一定位的意义尚不清楚。在异源内含子中，当NRS靠近真实的5‘剪接点时，其功能最佳。这一观察结果使我们研究了位置依赖的基础。提出了四种解释，并强调了参与剪接的三个主要元件：3‘剪接位点、5’剪接位点和5‘末端帽结构。NRS功能与其相对于3‘剪接点或帽结构的位置无关，而似乎依赖于其相对于真实5’剪接点的位置。我们得出结论，位置依赖性可能反映了NRS与真实的5‘剪接位点配对与3’剪接位点配对所需的距离限制。
Rous sarcoma virus (RSV) requires incomplete splicing of its viral transcripts to maintain efficient replication. A splicing inhibitor element, the negative regulator of splicing (NRS), is located near the 5′ end of the RNA but the significance of this positioning is not known. In a heterologous intron the NRS functions optimally when positioned close to the authentic 5′ splice site. This observation led us to investigate the basis of the position dependence. Four explanations were put forth and stressed the role of three major elements involved in splicing, the 3′ splice site, the 5′ splice site, and the 5′ end cap structure. NRS function was unrelated to its position relative to the 3′ splice site or the cap structure and appeared to depend on its position relative to the authentic 5′ splice site. We conclude that position dependence may reflect distance constraints necessary for competition of the NRS with the authentic 5′ splice site for pairing with the 3′ splice sites.