Protective immunity of recombinant Mycobacterium bovis BCG expressing rhomboid gene against Eimeria tenella challenge
Protective immunity of recombinant Mycobacterium bovis BCG expressing rhomboid gene against Eimeria tenella challenge
复制标题
表达菱形基因的重组牛分枝杆菌卡介苗对柔嫩艾美耳球虫攻击的保护性免疫。
DOI：
10.1016/j.vetpar.2008.11.006
复制
发表时间：
2009-03-23
影响因子：
2.6
通讯作者：
Zhang, Guocai
中科院分区：
文献类型：
--
作者：
Wang, Qiuyue;Li, Jianhua;Zhang, Guocai
用染色体外载体pMV261和整合载体pMV361携带柔嫩艾美耳球虫Rho基因的重组牛分枝杆菌卡介苗(RBCG)对鸡的免疫保护作用进行了研究。用卡介苗、重组卡介苗pMV261-Rho或重组卡介苗pMV361-Rho滴鼻免疫鸡，每隔2周免疫一次。重组卡介苗免疫雏鸡均能产生特异性免疫应答，且免疫鸡的CD4(+)和CD8(+)细胞百分率均显著高于对照组(P<；0.05)。攻毒实验表明，这两株重组卡介苗对柔嫩艾美耳球虫的攻毒具有明显的保护作用。但rBCG pMV261-Rho免疫组的特异性抗体滴度和保护率(56.04%)均高于rBCG pMV361 Rho组(P<0.05)。这些结果表明，牛分枝杆菌卡介苗是一种新型的表达和呈递柔嫩艾美耳球虫抗原的疫苗载体，重组卡介苗在鸡体内有潜在的疫苗应用前景。(C)2008爱思唯尔B.V.保留所有权利。
Two recombinant Mycobacterium bovis BCG (rBCG) strains carrying the Eimeria tenella rhomboid gene (Rho) delivered by extrachromosomal vector pMV261 and integrative vector pMV361 were evaluated for their ability to protect chickens against E tenella challenge. The chickens were immunized intranasal with BCG, rBCG pMV261-Rho, or rBCG pMV361-Rho twice at a 2-week interval. All the recombinant BCG immunized chickens developed specific immune responses, and there was a significant increases of the percentages of CD4(+) and CD8(+) cells compared to the control (P < 0.05). Challenge experiments demonstrated that the two rBCG strains could provide significant protection against E. tenella challenge. But vaccination with rBCG pMV261-Rho induced higher specific antibody titers and produced greater protection rate (56.04%) than rBCG pMV361 Rho group (P < 0.05). These results indicated that M. bovis BCG is a novel vaccine vector to express and present antigens of E. tenella, and rBCG has a potential as vaccine in chickens. (C) 2008 Elsevier B.V. All rights reserved.