Microwave CO2 plasma-initiated vapour phase graft polymerization of acrylic acid onto polytetrafluoroethylene for immobilization of human thrombomodulin
Microwave CO2 plasma-initiated vapour phase graft polymerization of acrylic acid onto polytetrafluoroethylene for immobilization of human thrombomodulin
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DOI：
10.1016/s0142-9612(97)00045-8
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发表时间：
1997-09-01
期刊：
影响因子：
14
通讯作者：
Jacobasch, HJ
中科院分区：
文献类型：
--
作者：
Vasilets, VN;Hermel, G;Jacobasch, HJ
通过 CO2 等离子体活化和随后的丙烯酸 (AA) 气相接枝聚合，实现了聚四氟乙烯 (PTFE) 与人血栓调节蛋白 (hTM) 结合的功能化。经过 CO2 等离子体处理、AA 接枝和 hTM 固定后的 PTFE 表面通过衰减全反射傅里叶变换红外 (ATR-FTIR) 光谱、X 射线光电子能谱 (XPS) 和电子自旋共振 (ESR) 光谱进行表征，并通过 zeta 电位和润湿测量来定量控制改性的每个步骤。通过蛋白C激活测试评估固定化hTM的活性。 (C) 1997 爱思唯尔科学有限公司。版权所有。
The functionalization of polytetrafluoroethylene (PTFE) for human thrombomodulin (hTM) binding has been achieved by CO2 plasma activation and subsequent vapour phase graft polymerization of acrylic acid (AA). The PTFE surfaces after CO2 plasma treatment, AA grafting and hTM immobilization were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and electron spin resonance (ESR) spectroscopy, as well as by zeta potential and wetting measurements to quantitatively control each step of modification. The activity of immobilized hTM was estimated by the protein C activation test. (C) 1997 Elsevier Science Limited. All rights reserved.