Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells
Identification of Zfp-57 as a downstream molecule of STAT3 and Oct-3/4 in embryonic stem cells
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DOI：
10.1016/j.bbrc.2005.03.118
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发表时间：
2005-05-27
影响因子：
3.1
通讯作者：
Yokota, T
中科院分区：
文献类型：
--
作者：
Akagi, T;Usuda, M;Yokota, T
胚胎干细胞（ES）是来源于囊胚内细胞群的多能细胞。转录因子STAT3对胚胎干细胞的自我更新至关重要。在本研究中，我们在ES细胞中寻找STAT3的下游分子。通过DNA芯片分析，获得锌指蛋白(Zfp)-57。Zfp-57的表达仅限于未分化的ES细胞，STAT3的激活导致Zfp-57的表达。我们还发现，强迫表达STAT3的显性阴性突变体或抑制Oct-3/4的表达会导致Zfp-57的下调。Zfp-57的靶向破坏没有导致明显的表型缺陷，包括未分化状态特异性基因的表达。这些数据表明，Zfp-57在ES细胞中是STAT3和Oct-3/4的下游分子，尽管对于它们的自我更新是必不可少的。(c) 2005爱思唯尔公司版权所有。
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor STAT3 is essential for the self-renewal of ES cells. In this study, we searched for downstream molecules of STAT3 in ES cells. Using DNA chip analysis, we obtained zinc finger protein (Zfp)-57. The expression of Zfp-57 was restricted to undifferentiated ES cells and activation of STAT3 led to expression of Zfp-57. We also found that forced expression of a dominant-negative mutant of STAT3 or repression of Oct-3/4 expression led to down-regulation of Zfp-57. Targeted disruption of Zfp-57 resulted in no gross phenotypical defects, including expression of undifferentiated-state-specific genes. These data suggest that Zfp-57 is a downstream molecule of STAT3 and Oct-3/4 in ES cells, although dispensable for their self-renewal. (c) 2005 Elsevier Inc. All rights reserved.