Hepatitis B virus DNA in the fingernails and hair of children with acute hepatitis B
Hepatitis B virus DNA in the fingernails and hair of children with acute hepatitis B
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DOI：
10.1016/j.jiac.2021.08.014
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发表时间：
2021-11-14
影响因子：
2.2
通讯作者：
Fujisawa, Tomoo
中科院分区：
文献类型：
--
作者：
Harada, Takumi;Komatsu, Haruki;Fujisawa, Tomoo
在慢性 HBV 感染患者的指甲和头发中可检测到乙型肝炎病毒 (HBV) DNA。然而，目前尚不清楚急性乙型肝炎感染患者的指甲和头发中是否可以检测到乙型肝炎病毒DNA。我们遇到了两例急性乙肝病毒感染的儿童。通过实时 PCR 评估了两个孩子指甲和头发中的 HBV DNA。为了阐明 HBV DNA 的特征，进行了全长 HBV 基因组测序和系统发育树分析。病例1和病例2儿童第0天的血清HBV DNA水平分别为7.6 Log IU/mL和7.4 Log IU/mL。两名儿童均检测到指甲 HBV DNA（病例 1：第 0 天为 4.6 Log IU/mL，病例 2：第 14 天为 5.5 Log IU/mL）。此外，病例 2 中可检测到头发 HBV DNA（第 14 天为 4.0 Log IU/mL）。第一次医院就诊后大约 3-4 个月内，血清 HBV DNA 检测不到。乙型肝炎病毒血症消退后，指甲和头发的乙型肝炎病毒 DNA 检测不到。血清、指甲和头发 HBV DNA 序列分析显示各病例的 HBV 基因型相同（病例 1：C 基因型，病例 2：A 基因型）。在案例 1 中，血清和指甲之间的全基因组 HBV 序列有 3 个核苷酸不同。在病例 2 中，血清、指甲和头发的全基因组 HBV 序列是相同的。总之，在急性 HBV 感染儿童的指甲和头发中可检测到 HBV DNA。
Hepatitis B virus (HBV) DNA is detectable in the nails and hair of patients with chronic HBV infection. However, it remains unclear whether HBV DNA can be detectable in the nails and hair of patients with acute HBV infection. We encountered two cases of children with acute HBV infection. HBV DNA in the nails and hair from the two children was evaluated by real-time PCR. To clarify the characteristics of HBV DNA, full-length HBV genome sequencing and phylogenetic tree analysis were performed. The levels of serum HBV DNA in children of cases 1 and 2 at day 0 were 7.6 Log IU/mL and 7.4 Log IU/mL, respectively. Nail HBV DNA was detected in both children (case 1: 4.6 Log IU/mL at day 0, case 2: 5.5 Log IU/mL at day 14). Moreover, hair HBV DNA was detectable in case 2 (4.0 Log IU/mL at day 14). Serum HBV DNA became undetectable within approximately 3-4 months after the first hospital visit. After the resolution of HBV viremia, nail and hair HBV DNA became undetectable. The sequence analysis of serum, nail and hair HBV DNA showed the same HBV genotype in each case (case1: genotype C, case 2: genotype A). In case 1, 3 nucleotides were different in the full-genome HBV sequence between the serum and nails. In case 2, the full-genome HBV sequences were identical among the serum, nails and hair. In conclusion, HBV DNA was detectable in nails and hair of children with acute HBV infection.