Ultrastructural analysis of polysialylated neural cell adhesion molecule in the suprachiasmatic nuclei of the adult mouse
Ultrastructural analysis of polysialylated neural cell adhesion molecule in the suprachiasmatic nuclei of the adult mouse
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成年小鼠视交叉上核聚唾液酸化神经细胞粘附分子的超微结构分析
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发表时间：
1999
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通讯作者：
Michiko Watanabe
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作者：
Huaming Shen;J. Glass;T. Seki;Michiko Watanabe
下丘脑前部的视交叉上核（SCN）被认为是哺乳动物的主要生物钟。成体SCN表达高水平的多唾液酸化神经细胞粘附分子（PSA-NCAM），这是一种能够调节细胞间相互作用的细胞表面唾液酸糖蛋白。在本研究中，通过使用针对PSA-NCAM的聚唾液酸（PSA）部分的单克隆抗体进行免疫标记，在超微结构水平上研究了PSA-NCAM在小鼠SCN中的表达。我们发现，PSA-NCAM的神经元体表达在SCN中分布不均匀，在SCN的中心区域有广泛的体染色，在核的腹侧部分有最小的体染色。相反，免疫反应性神经元，包括无髓细轴突束分布在整个SCN。还通过免疫过氧化物酶和免疫金技术在突触连接附近检测到PSA‐NCAM。对于星形胶质细胞，胞体和较大突起的免疫染色稀疏，但染色丰富，沿着细突起。免疫染色的精细星形胶质细胞的过程中经常观察到并置神经元胞体之间，并与突触连接和小血管密切相关。这些发现，以及PSA-NCAM在调节其他脑区细胞间相互作用中的作用，支持PSA-NCAM在调节SCN中细胞间相互作用中的作用。Anat Rec 256：448-457，1999年。© 1999 Wiley‐利斯公司
The suprachiasmatic nuclei (SCN) of the anterior hypothalamus are recognized as the principal circadian clock in mammals. The adult SCN express a high level of polysialylated neural cell adhesion molecule (PSA‐NCAM), a cell surface sialoglycoprotein capable of modulating cell‐cell interactions. In the present study, the expression of PSA‐NCAM in the mouse SCN was studied at the ultrastructural level by immunolabeling using monoclonal antibodies against the polysialic acid (PSA) moiety of PSA‐NCAM. We showed that neuronal somal expression of PSA‐NCAM was distributed heterogeneously in the SCN, with extensive staining of somas in the central region of the SCN, and minimal somal staining in the ventral portion of the nuclei. In contrast, immunoreactive neuropil, including unmyelinated fine axon fascicles was distributed throughout the SCN. The PSA‐NCAM was also detected adjacent to synaptic junctions by both immunoperoxidase and immunogold techniques. For astrocytes, immunostaining of somas and larger processes was sparse, but staining was profuse along fine processes. Immunostained fine astrocytic processes were frequently observed between apposing neuronal somas, and in close association with synaptic junctions and small blood vessels. These findings, together with the demonstrated role of PSA‐NCAM in modulating cell‐cell interactions in other brain regions support a role for PSA‐NCAM in regulating cell‐cell interactions in the SCN. Anat Rec 256:448–457, 1999. © 1999 Wiley‐Liss, Inc.