The Versatile Biocatalyst of Cytochrome P450 CYP102A1: Structure, Function, and Engineering.
The Versatile Biocatalyst of Cytochrome P450 CYP102A1: Structure, Function, and Engineering.
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细胞色素 P450 CYP102A1 的多功能生物催化剂：结构、功能和工程。
DOI：
10.3390/molecules28145353
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发表时间：
2023-07-12
期刊：
影响因子：
4.6
通讯作者：
Zhang, Haoming
中科院分区：
文献类型：
--
作者：
Sun, Yudong;Huang, Xiaoqiang;Osawa, Yoichi;Chen, Yuqing Eugene;Zhang, Haoming
来自巨型芽孢杆菌的野生型细胞色素P450 CYP102A1是一种高效的单加氧酶，用于氧化长链脂肪酸。CYP102A1具有高催化活性、表达量、区域选择性和立体选择性以及作为融合蛋白的电子转移自给自足等特点，是理想的生物催化剂。在过去的三十年中，CYP102A1在药物发现、生物合成和生物技术方面的应用取得了显著的进展。工程CYP102A1变异体的种类急剧增加，而底物种类则急剧增加，包括烷烃、烯烃、芳烃、有机溶剂、药品、药物等等。在这篇文章中，我们重点介绍了在过去五年中我们对CYP102A1的结构和功能的理解以及用于设计CYP102A1的新应用的方法的主要进展。目的是简要回顾cyp102a1体相关文献的最新进展。
Wild-type cytochrome P450 CYP102A1 from Bacillus megaterium is a highly efficient monooxygenase for the oxidation of long-chain fatty acids. The unique features of CYP102A1, such as high catalytic activity, expression yield, regio- and stereoselectivity, and self-sufficiency in electron transfer as a fusion protein, afford the requirements for an ideal biocatalyst. In the past three decades, remarkable progress has been made in engineering CYP102A1 for applications in drug discovery, biosynthesis, and biotechnology. The repertoire of engineered CYP102A1 variants has grown tremendously, whereas the substrate repertoire is avalanched to encompass alkanes, alkenes, aromatics, organic solvents, pharmaceuticals, drugs, and many more. In this article, we highlight the major advances in the past five years in our understanding of the structure and function of CYP102A1 and the methodologies used to engineer CYP102A1 for novel applications. The objective is to provide a succinct review of the latest developments with reference to the body of CYP102A1-related literature.
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