Cytokine release by activated T-cells in large granular lymphocytic leukemia associated with autoimmune disorders.
Cytokine release by activated T-cells in large granular lymphocytic leukemia associated with autoimmune disorders.
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DOI：
10.1038/sj.thj.6200149
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
L. Shvidel;Chen Duksin;Alexandra Tzimanis;M. Shtalrid;A. Klepfish;E. Sigler;M. Haran;E. Eilat;A. Berrebi
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文献类型：
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作者：
L. Shvidel;Chen Duksin;Alexandra Tzimanis;M. Shtalrid;A. Klepfish;E. Sigler;M. Haran;E. Eilat;A. Berrebi
前言T细胞大颗粒淋巴细胞白血病（T-LGLL）患者有很高的自身免疫性疾病发生率。相关的T-LGLL和自身免疫异常的发病机制尚不清楚。在这项研究中，我们已经调查了细胞因子在LGLL.Patients和METHODS免疫并发症的发展中的作用，我们研究了15例诊断为T-LGLL患者的临床和实验室特征。患者有各种自身免疫性疾病：持续性中性粒细胞减少症，免疫性血小板减少症，纯红细胞再生障碍性贫血，桥本甲状腺炎，干燥综合征，系统性红斑狼疮，系统性硬皮病。从这些患者获得的T-LGLL细胞通过植物血凝素活化并孵育3天。用ELISA法检测了细胞培养上清中sIL-2 R、IL-4、IL-6、IL-8、IL-10、IL-12和TNF-α的释放，用PHA刺激的LGLL T细胞培养上清中sIL-2 R的分泌增加了40%（P <0.01）。在所有患者中，TNF-α-（6名患者）、TNF-α-（47%）（7名患者）、IL-6-（67%）（10名患者）、IL-10-（47%）（7名患者）和IL-8-（60%）（9名患者）。与对照组相比，IL-4和IL-12水平未升高。LGL计数，CD 4与CD 8扩增，或在患者的临床表现和细胞因子释放在vitro.CONCLUSIONOUR的研究结果表明，潜在的LGLL细胞的细胞因子释放在体外表明，这些细胞可能发挥重要作用，在大颗粒淋巴细胞白血病伴随自身免疫功能的免疫紊乱中观察到。
INTRODUCTIONPatients with T-cell large granular lymphocytic leukemia (T-LGLL) have a high incidence of autoimmune disorders. The pathogenesis of associated T-LGLL and autoimmune abnormalities is not clear. In this study we have investigated the role of cytokines in the development of immune complications in LGLL.PATIENTS AND METHODSWe studied clinical and laboratory features of 15 patients diagnosed with T-LGLL. The patients had various autoimmune disturbances: persistent neutropenia, immune thrombocytopenia, pure red-cell aplasia, Hashimoto's thyroiditis, sicca syndrome, systemic lupus erythemathosus, systemic scleroderma. The T-LGLL cells obtained from these patients were activated by phytohemagglutinin and incubated for 3 days. Using ELISA technique we analysed the release of sIL-2R, IL-4, IL-6, IL-8, IL-10, IL-12 and TNF-alpha in the supernatant.RESULTSCytokine analysis of supernatants obtained from the LGLL T cells stimulated with PHA revealed increased sIL-2R production in 40%(six patients), TNF-alpha-in 47%(seven patients), IL-6-in 67%(10 patients), IL-10-in 47%(seven) and IL-8-in 60%(nine) of patients. Levels of IL-4 and IL-12 were not elevated compared to controls. No correlation was found between LGL count, CD4 versus CD8 expansion, or in the clinical findings of the patients and cytokine release in vitro.CONCLUSIONOur findings showing the potential of LGLL cells for cytokine release in vitro suggests that these cells may play a major role in the immune disturbances observed in large granular lymphocytic leukemia accompanied by autoimmunity features.