STUDY OF DIFFERENTIAL-EFFECTS OF KAINIC ACID ON METABOLIC RATES, UTILIZING EXOGENOUS OR ENDOGENOUS SUBSTRATES, IN RAT-BRAIN SLICES
STUDY OF DIFFERENTIAL-EFFECTS OF KAINIC ACID ON METABOLIC RATES, UTILIZING EXOGENOUS OR ENDOGENOUS SUBSTRATES, IN RAT-BRAIN SLICES
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DOI：
10.1111/j.1471-4159.1983.tb09042.x
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发表时间：
1983-01-01
影响因子：
4.7
通讯作者：
BARNABEI, O
中科院分区：
文献类型：
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作者：
POLI, A;MIGANI, P;BARNABEI, O
在不同浓度的红藻氨酸存在下，测定皮质、整个海马和CA 3区海马脑片从外源性葡萄糖产生的CO2，以及整个海马脑片的O2消耗。在10-4-10-2 M的红藻氨酸浓度下，仅在CA 3区海马制备物中观察到CO2产生的中度显著增加。O2的消耗，在整个海马切片的内源性能量储存的费用，大大增加了10-3 M红藻氨酸的切片孵育时，没有外源性葡萄糖。这种效应部分地被高K+浓度（50 mM）所抵消。一些可能的因素参与脑切片的差异代谢反应的作用红藻氨酸进行了简要的讨论。
CO2 production from exogenous glucose of cortical, whole hippocampal and CA3 region hippocampal slices, as well as O2 consumption of whole hippocampal slices, were measured in the presence of different concentrations of kainic acid. A moderate, significant increase of CO2 production was seen only in the CA3 region hippocampal preparation at kainic acid concentrations of 10-4-10-2 M. The O2 consumption, at the expense of endogenous energy stores of whole hippocampal slices, was substantially increased by 10-3 M kainic acid when the slices were incubated without exogenous glucose. The effect was partly paralleled by the use of high (50 mM) K+ concentration. Some of the possible factors involved in the differential metabolic responses of brain slices to the action of kainic acid are discussed briefly.