PHENOTYPIC SELECTION OF SMALL-COLONY VARIANT FORMS OF STAPHYLOCOCCUS-EPIDERMIDIS IN THE RAT MODEL OF ENDOCARDITIS
PHENOTYPIC SELECTION OF SMALL-COLONY VARIANT FORMS OF STAPHYLOCOCCUS-EPIDERMIDIS IN THE RAT MODEL OF ENDOCARDITIS
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DOI：
10.1093/infdis/157.4.757
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发表时间：
1988-04-01
影响因子：
6.4
通讯作者：
CHRISTENSEN, GD
中科院分区：
文献类型：
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作者：
BADDOUR, LM;SIMPSON, WA;CHRISTENSEN, GD
表皮葡萄球菌的四种致病菌株-EC、RP 62 A、LW和HBSN-在高盐、低葡萄糖琼脂（“孟菲斯琼脂”）上铺板时表现出菌落表型的混合群体。当与挑战接种相比时，对于从导管诱导的心内膜炎大鼠感染赘生物中回收的所有4种菌株，菌落群向小菌落变体形式发生显著变化。采用大鼠心内膜炎模型，比较了HBSN株的各种菌落表型在毒力研究中的差异。小菌落变异型的感染率显著低于正常亲本型（P <0.05）或混合表型接种物（P <0.05）。这些数据表明，一旦确定心内膜炎感染，可以选择小菌落变异型，并且单独的小菌落变异型在实验性心内膜炎中启动和/或维持心内感染的能力要小得多。
Four pathogenic strains of Staphylococcus epidermidis (sensu strictu)-EC, RP62A, LW, and HBSN-exhibited a mixed population of colony phenotypes when plated onto a high-salt, low-glucose agar ("Memphis agar"). When compared with challenge inocula, significant shifts in colony populations to the small-colony variant forms occurred for all four strains for isolates recovered from infected vegetations of rats with catheter-induced endocarditis. Various colony phenotypes of the HBSN strain were compared in virulence studies by using the rat model of endocarditis. The infectivity rate of the small-colony variant forms was significantly less than that of either the normal parent form (P < .05) or the mixed phenotypic inoculum (P < .05). These data indicate that the small-colony variant forms may be selected for, once endocardial infections are established, and that the small-colony variant forms alone are much less able to initiate and/or sustain intracardiac infections in experimental endocarditis.